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Studies on the alkalophilicStreptomyces with extracellular xylanolytic activity

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Summary

An alkalophilicStreptomyces which produced xylanase, isolated from soil, grew in a temperature range of 15–37°C. The pH optimum for growth was 10 and no growth occurred at pH 7. On a simple wheat bran medium the microorganism exhibited maximum enzyme secretion of 12 U/ml at pH 10. The enzyme had a broad pH optimum of 4.8–10 and the optimum temperature of 50°C. It was completely inactivated at 60°C in 2 h. The enzyme hydrolyzed xylan to a mixture of oligomeric products indicating that the main activity was of the endoxylanase type. The culture filtrate had no cellulase activity.

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References

  • Berghem, L. E. R. and Pettersson, L. G. (1973)Eur. J. Biochem. 37, 21–30.

    Google Scholar 

  • Biely, P. (1985)Trends in Biotechnol. 3, 286–290.

    Google Scholar 

  • Crawford, D. L. (1981)Biotechnol. Bioeng. Symp. 11, 275–291.

    Google Scholar 

  • Davis, B. J. (1964)Ann. N.Y. Acad. Sci. 121 404–427.

    Google Scholar 

  • Dekker, R. F. H. and Richard, J. N.Advances in Carbohydrate Chemistry, Academic Press, London (1976), pp. 278–352.

    Google Scholar 

  • Honda, H., Kudo, T., Ikura, Y. and Horikoshi, K. (1985)Can. J. Microbiol. 31, 538–542.

    Google Scholar 

  • Ishaque, M. and Klupfel, F. (1980)Can. J. Microbiol. 26, 183–189.

    Google Scholar 

  • Klupfel, D., Shareack, F., Mondou, F. and Morosoli, R. (1986)Appl. Microbiol. Biotechnol. 24, 230–234.

    Google Scholar 

  • Mandels, M. and Weber, J. (1969)Adv. Chem. Ser. 95, 395.

    Google Scholar 

  • McCarthy, A. J., Peace, E. and Broda, P. (1985)Appl. Microbiol. Biotechnol. 21, 238–244.

    Google Scholar 

  • Miller, G. L. (1959)Anal. Chem. 31, 426–428.

    Google Scholar 

  • Okazaki, W., Akiba, T., Horikoshi, K. and Akahoshi, R. (1984)Appl. Microbiol. Biotechnol. 19, 335–340.

    Google Scholar 

  • Reisfeld, R. A., Lewis, U. J. and Williams, D. E. (1962)Nature 195, 281–283.

    Google Scholar 

  • Trevelyn, W. E., Procter, D. P. and Harrison, J. S. (1950)Nature, 166, 444–445.

    Google Scholar 

  • Van Zyl, W. H. (1985)Biotechnol. Bioeng. 27, 1367–1373.

    Google Scholar 

  • Wong, K. K. Y., Tan, L. U. L. and Saddler, J. N. (1988)Microbiol. Reviews, 52, 305–317.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, 411008, Pune, India

    P. Vyas, V. Chauthaiwale, S. Phadatare, V. Deshpande & M. C. Srinivasan

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  1. P. Vyas
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  2. V. Chauthaiwale
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  3. S. Phadatare
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  4. V. Deshpande
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  5. M. C. Srinivasan
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Vyas, P., Chauthaiwale, V., Phadatare, S. et al. Studies on the alkalophilicStreptomyces with extracellular xylanolytic activity. Biotechnol Lett 12, 225–228 (1990). https://doi.org/10.1007/BF01026804

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