Biotechnology Letters

, Volume 11, Issue 1, pp 49–52 | Cite as

Solid state fermentation for production of higher titres of thermostable alpha-amylase with two peaks for pH optima byBacillus licheniformis M27

  • M. V. Ramesh
  • B. K. Lonsane
Article

Summary

Bacillus licheniformis M27 produced 21, 000 units of alpha-amylase/g dry bacterial bran under solid state fermentation in wheat bran medium enriched with 3.3% di-ammonium hydrogen phosphate. The crude enzyme, with temperature optimum at 90°C in 0.5% starch solution, showed pH optima at 6.5–7.0 and 9.5 and over 75% activity over the pH range 6.0–10.5.

Keywords

Fermentation Starch Bacillus Bioorganic Chemistry Bran 

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References

  1. Aunstrup, K. (1983). Enzymes of industrial interest-Traditional Products. In:Annual Reports on Fermentation Processes, G.T. Tsao, ed. Vol. 6, pp. 175–201. New York: Academic Press.Google Scholar
  2. Aunstrup, K., Anderson, O., Falch, E.A. and Nielsen, T.K. (1979). Production of microbial enzymes. In:Microbial Technology, Vol. I, 2nd ed. H.J. Peppler and D. Perlman eds., pp. 281–309. New York: Academic Press.Google Scholar
  3. Bernfeld, P. (1955).Methods in Enzymol. 1, 149–158.Google Scholar
  4. Board of Science and Technology for International Development (1982).Priorities in Biotechnology Research for International Development: Proceedings in a Workshop, pp. 13–14. Washington D.C.: National Academy Press.Google Scholar
  5. Ghildyal, N.P., Lonsane, B.K., Sreekantian, K.R. and Murthy, V.S. (1985).J. Fd. Sci. Technol. 22, 171–176.Google Scholar
  6. Gibson, T. and Gordon, R.E. (1974). Genus I.Bacillus. In:Bergey's Manual of Determinative Bacteriology, R.E. Buchanan and N.E. Gibbons co-eds., ed.8, pp. 529–550. Baltimore: The Williams and Wilkins Co.Google Scholar
  7. Gordon, R.E. (1977). The genusBacillus. In:CRC Handbook of Microbiology, A.I.Laskin and H.A.Lechevalier, eds. Vol. 1, pp. 319–336. Ohio: CRC Press Inc.Google Scholar
  8. Grueninger, H., Sonnleitner, B. and Fiechter, A. (1984).Appl. Microbiol. Biotechnol. 19, 414–421.Google Scholar
  9. Lonsane, B.K., Ghildyal, N.P., Budiatman, S. and Ramakrishna, S.V. (1985).Enz. Microbial Technol. 7, 258–265.Google Scholar
  10. Ramesh, M.V. and Lonsane, B.K. (1987a).Biotechnol. Letts. 9, 323–328.Google Scholar
  11. Ramesh, M.V. and Lonsane, B.K. (1987b).Biotechnol. Letts. 9, 501–504.Google Scholar
  12. Society of American Bacteriologists (1957).Manual of Microbiological Methods. H.Conn ed. New York: McGraw Hill Book Co. Inc.Google Scholar
  13. Trevelyan, W.E., Proctor, D.P. and Harrison, J.S. (1950).Nature, London 166, 444–445.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • M. V. Ramesh
    • 1
  • B. K. Lonsane
    • 1
  1. 1.Fermentation Technology and Bioengineering DisciplineCentral Food Technological Research InstituteMysoreIndia

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