Biotechnology Letters

, Volume 11, Issue 1, pp 49–52 | Cite as

Solid state fermentation for production of higher titres of thermostable alpha-amylase with two peaks for pH optima byBacillus licheniformis M27

  • M. V. Ramesh
  • B. K. Lonsane


Bacillus licheniformis M27 produced 21, 000 units of alpha-amylase/g dry bacterial bran under solid state fermentation in wheat bran medium enriched with 3.3% di-ammonium hydrogen phosphate. The crude enzyme, with temperature optimum at 90°C in 0.5% starch solution, showed pH optima at 6.5–7.0 and 9.5 and over 75% activity over the pH range 6.0–10.5.


Fermentation Starch Bacillus Bioorganic Chemistry Bran 
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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • M. V. Ramesh
    • 1
  • B. K. Lonsane
    • 1
  1. 1.Fermentation Technology and Bioengineering DisciplineCentral Food Technological Research InstituteMysoreIndia

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