Summary
The cells ofCitrobacter intermedius (containing L-tyrosine phenol lyase which catalyses the synthesis of 3-fluoro-L-tyrosine fromo-fluorophenol, pyruvate and ammonia) were entrapped into 10% poly(vinyl alcohol) cryogel beads. Cryogenic immobilization resulted in a moderate increase of the synthetic activity of the cells. During continuous operation for one month in a packed bed column reactor the productivity of the immobilized biocatalyst decreased by 30%.
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Lozinsky, V.I., Faleev, N.G., Zubov, A.L. et al. Use of PVA-cryogel entrappedCitrobacter intermedius cells for continuous production of 3-fluoro-L-tyrosine. Biotechnol Lett 11, 43–48 (1989). https://doi.org/10.1007/BF01026784
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DOI: https://doi.org/10.1007/BF01026784