The effect of oxygen limitation on stability of a recombinant plasmid inSaccharomyces cerevisiae
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The effect of oxygen limitation on the stability of a recombinant yeast plasmid was measured during continuous culture in a non-selective medium. The fraction of plasmid containing cells was found to decrease more rapidly after a step change to low dissolved oxygen (DOT) levels. An increased rate of plasmid loss appeared to be the major cause of the decreased stability.
KeywordsOxygen Organic Chemistry Dissolve Oxygen Bioorganic Chemistry Recombinant Plasmid
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