Biotechnology Letters

, Volume 11, Issue 1, pp 5–10 | Cite as

The effect of oxygen limitation on stability of a recombinant plasmid inSaccharomyces cerevisiae

  • P. Caunt
  • A. Impoolsup
  • P. F. Greenfield
Article

Summary

The effect of oxygen limitation on the stability of a recombinant yeast plasmid was measured during continuous culture in a non-selective medium. The fraction of plasmid containing cells was found to decrease more rapidly after a step change to low dissolved oxygen (DOT) levels. An increased rate of plasmid loss appeared to be the major cause of the decreased stability.

Keywords

Oxygen Organic Chemistry Dissolve Oxygen Bioorganic Chemistry Recombinant Plasmid 

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • P. Caunt
    • 1
  • A. Impoolsup
    • 1
  • P. F. Greenfield
    • 1
  1. 1.Centre for Molecular Biology and BiotechnologyUniversity of QueenslandSt. LuciaAustralia

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