The effect of oxygen limitation on stability of a recombinant plasmid inSaccharomyces cerevisiae
The effect of oxygen limitation on the stability of a recombinant yeast plasmid was measured during continuous culture in a non-selective medium. The fraction of plasmid containing cells was found to decrease more rapidly after a step change to low dissolved oxygen (DOT) levels. An increased rate of plasmid loss appeared to be the major cause of the decreased stability.
KeywordsOxygen Organic Chemistry Dissolve Oxygen Bioorganic Chemistry Recombinant Plasmid
Unable to display preview. Download preview PDF.
- Barnett, J.A., Payne, R.W. and Yarrow, D. (1983).Yeasts: characteristics and identification. Cambridge University Press.Google Scholar
- DiBiasio, D. and Sardonini, C. (1986).Ann. N. Y. Acad. Sci. 469, 111–117.Google Scholar
- Cooper, N.S., Brown, M.E. and Caulcott, C.A. (1987).J. Gen. Microbiol. 133, 1871–1880.Google Scholar
- Furukawa, K., Heinzle, E. and Dunn, I.J. (1983).Biotechnol. Bioeng. 25, 2293–2317.Google Scholar
- Futcher, A.B. and Cox, B.S. (1984).J. Bacteriol. 157, 283–290.Google Scholar
- Guarente, L. and Ptashne, M. (1981).Proc. Natl. Acad. Sci. 78, 2199–2203.Google Scholar
- Impoolsup, A., Caunt, P. and Greenfield, P.F. (1988). Manuscript in preparation.Google Scholar
- Kleinman, M.J., Gingold, E.B. and Stanbury, P.F. (1986).Biotechnol. Lett. 8, 225–230.Google Scholar
- Lee, F.J. and Hassan, H.M. (1987).Appl. Microbiol. Biotechnol. 27, 72–74.Google Scholar
- Miller, J.H. (1972). Assay of β-galactosidase. In:Experiments in Molecular Genetics, J.H. Miller, ed. pp. 352–355. New York: Cold Spring Harbor Laboratory.Google Scholar
- Tolentino, G.J. and San, K.Y. (1988),Biotechnol. Lett. 10, 373–376.Google Scholar