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Nicotine production by “hairy root” cultures of Nicotiana rustica: Fermentation and product recovery

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Abstract

The production of nicotine by cultures ofNicotiana rustica transformed withAgrobacterium rhizogenes has been examined in a packed bed fermenter as a two-stage batch/continuous-flow system. A substantial proportion of the nicotine synthesised in the batch phase may be subsequently harvested from the medium. The possibility of improving product recovery using macroreticular adsorbents is considered.

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References

  1. Deus-Neumann, B. and Zenk, M.H. (1984) Planta Medica,50, 427–431.

    Google Scholar 

  2. Hamill, J.D., Parr, A.J., Robins, R.J. and Rhodes, M.J.C. (1986) Plant Cell Reports (in press).

  3. Martin, S.M. (1980) in Plant Tissue Culture as a source of Biochemicals (E.J. Staba, ed.) CRC Press Boca Raton, 143–148.

    Google Scholar 

  4. Morris, P. (1986) Proc. IAPTC (UK) Symposium, Sheffield 1985. (Morris, P., Scragg, A.H., Stafford, A. and Fowler, M.W. eds) Cambridge University Press.

  5. Mugnier, J., Jung, G. and Prious, J.-L. (1983) Europ. Patent No. EP 0 100 691 A1.

  6. Noguchi, M., Matsumoto, T., Hirata, Y., Yamamoto, K., Katsuyma, A., Kato, A., Azechi, S. and Kato, K. (1977) in Plant Tissue Culture and its Biotechnological Application (Barz, W., Reinhard, E. and Zenk, M.K. eds.) Springer-Verlag Berlin, Heidelberg, New York, pp. 85–94.

    Google Scholar 

  7. Robins, R.J. and Rhodes, M.J.C. (1986) Appl. Microbiol. Biotech. (in press).

  8. Tepfer, D. (1984) Cell37, 959–967.

    Google Scholar 

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Authors and Affiliations

  1. AFRC Institute of Food Research Norwich Laboratory), Colney Lane, NR4 7UA, Norwich

    M. J. C. Rhodes, M. Hilton, A. J. Parr, J. D. Hamill & R. J. Robins

Authors
  1. M. J. C. Rhodes
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  2. M. Hilton
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  3. A. J. Parr
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  4. J. D. Hamill
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  5. R. J. Robins
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Rhodes, M.J.C., Hilton, M., Parr, A.J. et al. Nicotine production by “hairy root” cultures of Nicotiana rustica: Fermentation and product recovery. Biotechnol Lett 8, 415–420 (1986). https://doi.org/10.1007/BF01026743

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  • DOI: https://doi.org/10.1007/BF01026743

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