Biotechnology Letters

, Volume 8, Issue 6, pp 391–396 | Cite as

Enhanced stability of a 2μ-based recombinant plasmid in diploid yeast

  • David J. Mead
  • David C. J. Gardner
  • Stephen G. Oliver


The stability of a 2μ-based recombinant plasmid, pJDB219, has been compared in glucose-limited chemostat cultures of two haploid strains ofSaccharomycescerevisiae and a diploid derived from them. The stability of the recombinant plasmid differed in the two haploid hosts but was greatest in the diploid. Enhanced stability in the diploid is probably a function of both the increased copy number and reduced selective burden of the plasmid.


Organic Chemistry Bioorganic Chemistry Recombinant Plasmid Chemostat Culture Increase Copy Number 
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Copyright information

© Kluwer Academic Publishers 1986

Authors and Affiliations

  • David J. Mead
    • 1
  • David C. J. Gardner
    • 1
  • Stephen G. Oliver
    • 1
  1. 1.Department of Biochemistry and Applied Molecular BiologyUniversity of Manchester Institute of Science and TechnologyManchesterEngland

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