Summary
The effects of pH, temperature and substrate concentration on the cellulase (B-1,4-endoglucanase) activity ofA. candidus were studied. Maximum enzyme activities were obtained when the concentration of the substrate (CMC) was 6 mg per ml, at pH 4, and temperature 50 C. The enzyme retained 85% of its original activity under optimal conditions of pH and temperature after 36 hours of incubation. The Km constant of the reaction was calculated as 2.3 mg of CMC per ml and the energy of activation of the enzyme as 7.92 k cal per mole.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Attia, R. M., and Gamal, R. F. (1980). Rev. Microbiol. 11, 64–70.
Dickerson, A. G., and Pollard, C. M. D. (1982). Physiol. Plant Pathol. 21, 179–192.
Fennington, G., Lupo, D., and Stutzenberger, F. (1982). Biotechnol. Bioeng. 24, 2487–2498.
Korkulanin, A., Hrasak, D., and Johanides, V. (1968). Mikrobiologiya 5, 163.
Mandels, M., and Weber, J. (1969). Adv. Chem. Ser. 95, 391–413.
Mandels, M. (1975). Biotechnol. Bioeng. Symp. No. 5, 81–105.
Ortega, J. (1980). Tex. J. Sci. 32, 241–246.
Ortega, J., and Baca, E. J. (1983). Tex. J. Sci. 35, 261–267.
Sternberg, D., and Mandels, G. R. (1979). J. Bacteriol. 139, 761–769.
Williams, V. R., and Williams, H. B. (1973). Basic physical chemistry for the life sciences. W. H. Freeman and Co., San Francisco. 313–320.
Workman, W. E., and Day, D. F. (1982). Appl. Environ. Microbiol. 44, 1289–1295.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ortega, J. Some characteristics of the cellulase of Aspergillus candidus. Biotechnol Lett 7, 109–112 (1985). https://doi.org/10.1007/BF01026680
Issue Date:
DOI: https://doi.org/10.1007/BF01026680