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Microbial reduction of ethyl acetoacetate using Sclerotium rolfsii

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Summary

A method for the quantitative enantioselective bioreduction of ethyl acetoacetate [1] to optically pure (+)-S-ethyl-3 hydroxybutyrate [II] usingSclerotium rolfsii mycelium is described. In a synthetic medium 1 g mycelium (dry weight) could convert 1 g of I to II within 2–3 days of fermentation (pH 5.8, 30°C). This is the first report demonstrating use ofS. rolfsii biomass for asymmetric reduction to get chiral building blocks.

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Authors and Affiliations

  1. Organic Chemistry, National Chemical Laboratory, 411008, Pune, India

    R. D. Wakharkar & B. Chanda

  2. Biochemical Sciences Division, National Chemical Laboratory, 411008, Pune, India

    A. H. Lachke

Authors
  1. R. D. Wakharkar
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  2. B. Chanda
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  3. A. H. Lachke
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Wakharkar, R.D., Chanda, B. & Lachke, A.H. Microbial reduction of ethyl acetoacetate using Sclerotium rolfsii. Biotechnol Lett 11, 483–486 (1989). https://doi.org/10.1007/BF01026646

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  • DOI: https://doi.org/10.1007/BF01026646

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