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Biotechnology Letters

, Volume 7, Issue 8, pp 593–596 | Cite as

Purification of glucose isomerase from Streptomyces nigrificans

  • B. Kralova
  • K. Demnerova
  • P. Skrivan
Article

Summary

A relatively simple method for obtaining an electrophoretically homogeneous preparation of glucose isomerase from Streptomyces nigrificans is described. Extract of disintegrated microbial cells was first heated at 60°C in the presence of Co and Mg ions. Centrifugation and ultrafiltration were followed by ion exchange chromatography on DEAE-cellulose. The fraction with glucose isomerase activity proved to contain no proteins other than the isolated enzyme.

Keywords

Glucose Enzyme Chromatography Purification Centrifugation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Demnerova, K., Pospisil, S., Valentova, O., Kas, J. (1979) Biotech. Lett, 1, 293–298.Google Scholar
  2. Demnerova, K., Fort, I., Kralova, B., Safarik, I., Benesova, I. (1984). Biotech. Lett. 6, 699–702.Google Scholar
  3. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). J. Biol. Chem. 193, 265–275.Google Scholar
  4. Safarik, I. (1983). J. Chromatog. 261, 138–141.Google Scholar

Copyright information

© Science and Technology Letters 1985

Authors and Affiliations

  • B. Kralova
    • 1
  • K. Demnerova
    • 1
  • P. Skrivan
    • 1
  1. 1.Department of Biochemistry and MicrobiologyInstitute of Chemical TechnologyPrague 6CSSR

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