Purification of glucose isomerase from Streptomyces nigrificans
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A relatively simple method for obtaining an electrophoretically homogeneous preparation of glucose isomerase from Streptomyces nigrificans is described. Extract of disintegrated microbial cells was first heated at 60°C in the presence of Co and Mg ions. Centrifugation and ultrafiltration were followed by ion exchange chromatography on DEAE-cellulose. The fraction with glucose isomerase activity proved to contain no proteins other than the isolated enzyme.
KeywordsGlucose Enzyme Chromatography Purification Centrifugation
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