Skip to main content
Log in

Use of liposomes in transforming yeast cells

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Plasmid DNAs of YEp13 and pMA56 were encapsulated in liposomes. Cells ofSaccharomyces cerevisiae strain SHY3 were protoplasted. After fusing membranes of the liposomes and the protoplasts, transformation of the regenerated yeast cells with the plasmids has been confirmed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anil, B. M., Sheldon, O., Jean, B. D., Timothy, T., Peter, L. and Joseph, S. D. (1978),Proc. Nat. Acad. Sci. U.S.A. 75, 1361–1365.

    Google Scholar 

  • Botstein, D., Falco, S. C., Stewart, S. E., Brennan, M., Scherer, S., Stinchomb, D. T., Struhl, K., and Davis, R. W. (1979),Gene, 8, 17–24.

    Google Scholar 

  • Deamer, D. and Bangham, A. D. (1976),Biochim. Biophys. Acta, 443, 629–634.

    Google Scholar 

  • Fraley, R. T., Fornari, C. S. and Kaplan, S. (1979),Proc. Natl. Acad. Sci. U.S.A. 76, 3348–3352.

    Google Scholar 

  • Fraley, R. T., Subramani, S., Berg, P. and Papahadjopoulos, D. (1980),J. Biochem. 255, 10431–10435.

    Google Scholar 

  • Gunge, N. and Tamaru, A. C. (1978),Japan J. Genetics, 53, 41–49.

    Google Scholar 

  • Lurquin, P. F. and Sheehy, R. E. (1982),Plant Science Letters, 25, 133–146.

    Google Scholar 

  • Makins, J. F. and Holt, G. (1981),Nature, 293, 671–673.

    Google Scholar 

  • Nagata, T., Okada, K., Takebe, I. and Matsuris, C. (1981),Mol. Gen. Gent. 184, 161–165.

    Google Scholar 

  • Ostro, M. J., Lavelle, D., Paxon, W., Mattews, B. and Giacomani, D. (1982),Arch. Biochim. Biophys. 201, 392–402.

    Google Scholar 

  • Rodicio, M. R. and Chater, K. F. (1982),J. Bacteriol. 151, 1078–1085.

    Google Scholar 

  • Sherman, F., Fink, G. R., Hicks, J. B. (1981),Methods in Yeast Genetics, 113–114, Cold Spring Harbor Laboratory, N. Y. U.S.A.

    Google Scholar 

  • Szoka, F. Jr. and Papahadjopoulos, D. (1978),Proc. Natl. Acad. Sci. U.S.A. 75, 4194–4198.

    Google Scholar 

  • van Solingen, P. and van der Plaat, J. B. (1977),J. Bacteriol. 130, 946–947.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ahn, J.S., Pack, M.Y. Use of liposomes in transforming yeast cells. Biotechnol Lett 7, 553–556 (1985). https://doi.org/10.1007/BF01026444

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01026444

Keywords

Navigation