Zusammenfassung
In vorausgehenden Untersuchungen war festgestellt worden, da\ die normalerweise Friend Virus-resistenten C 57 Bl/6-Knochenmarkzellen nach Transplantation in letal bestrahlte leukämische DBA/2-Mäuse virusempfindlich wurden. Dieser Resistenzverlust wurde weiter untersucht und das Milzgewicht sowie die Zahl der Ep-unabhängigen CFU-E als Resultat einer erfolgreichen FV-P-Infektion gemessen. C 57 Bl/6-Knochenmark wurde kurz vor und in Abständen nach isogeneischer Transplantation oder Transplantation in DBA/2-Mäuse infiziert. Nach isogeneischer Transplantation zeigte sich eine gewisse Empfindlichkeit für FV-P, und diese schien mit der regenerativen Aktivität der BFU-E zum Zeitpunkt der Infektion zu korrelieren. Es bestand jedoch kein Unterschied in der BFU-E-Kinetik zwischen normalen DBA/2- und C 57 Bl/6-Mäusen. C 57 Bl/6-Mäuse wurden weiterhin vorbehandelt durch Blutentnahme, Bestrahlung mit 150 R, Gabe von Hydroxyharnstoff und von Endotoxin. Nach Hydroxyharnstoff waren vermehrt BFU-E in der DNA-Synthesephase, aber nur nach Endotoxin, das nach 48 h keinen Einflu\ auf die BFU-E-Kinetik zeigte, waren die C 57 Bl/6-Mäuse empfindlich für die FV-P-Infektion. Diese Resultate legen nahe, da\ die proliferative Aktivität für eine erfolgreiche FV-P-Infektion und die Induktion einer Ep-unabhängigen Erythropoese wichtig ist. aber da\ au\erdem andere Bedingungen Resistenz bzw. Empfindlichkeit eines Mäusestammes bestimmen.
Summary
In previous studies it had been found that C 57 Bl/6 marrow cells, normally resistant to infection by the Friend virus (FV-P), became susceptible after their transplantation into lethally irradiated leukemic DBA/2 mice. This loss of FV-P resistance was further analyzed and spleen weight and the number of erythropoietin-(Ep)-independent CFU-E in bone marrow and spleen were measured as the result of successful FV-P infection. C 57 Bl/6 marrow was infected shortly before and in intervals after either isogeneic transplantation or transplantation into lethally irradiated DBA/2 hosts. After isogeneic transplantation there was some susceptibility for FV-P and this seemed to correlate with the regenerative activity of BFU-E at the time of infection. However, there was no difference in the BFU-E kinetics between normal DBA/2 and C 57 Bl/6 mice. C 57 Bl/6 were further pretreated by bleeding, irradiation with 150 R, injection of hydroxyurea and of endotoxin. After hydroxyurea treatment there was an increased proportion of BFU-E in S-phase, but only endotoxin, which did not alter the BFU-E kinetics after 48 h, made C 57 B1/6 mice susceptible to FV-P infection. These results suggest that the proliferative activity of BFU-E might be important for successful FV-P infection and induction of Ep-independent erythropoiesis but also other conditions regulate the resistance or susceptibility of a mouse strain.
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Supported by the Deutsche Forschungsgemeinschaft (SFB 112) and Stiftung Volkswagenwerk
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Seidel, H.J., Kreja, L. Studies on the resistance of the C 57 Bl/6 mouse strain to the polycythemia-inducing strain of the Friend virus (FV-P). Blut 42, 173–182 (1981). https://doi.org/10.1007/BF01026387
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DOI: https://doi.org/10.1007/BF01026387