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Biotechnology Letters

, Volume 14, Issue 2, pp 99–104 | Cite as

Biotechnological production of unnatural L-amino acids from D,L-5-monosubstituted hydantions. I. Derivatives of L-phenylalanine

  • Christoph Syldatk
  • Vera Lehmensiek
  • Gaby Ulrichs
  • Ulrich Bilitewski
  • Karsten Krohn
  • Hartmut Höke
  • Fritz Wagner
Article

Summary

Resting cells of a mutant ofArthrobacter sp. (DSM 3747) were used for the bioconversion of D,L-5-benzylhydantoin and related compounds to the corresponding L-amino acids. After optimization of the reaction conditions in shake flask experiments, bioconversions were performed in a preparative scale in a 2-l-bioreactor under nitrogen atmosphere. Specific productivities of 0.4 (p-NO2-L-phenylalanine) up to 3.9 mM amino acid x g cell dry mass−1 x h−1 (p-Cl-L-phenylalanine) were obtained. D,L-5-p-COOH-Benzylhydantoin, D,L-5-phenylhydantoin and D,L-5-p-OH-phenylhydantoin were not accepted as substrates.

Keywords

Nitrogen Atmosphere Organic Chemistry Bioorganic Chemistry Nitrogen Atmosphere 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Christoph Syldatk
    • 1
  • Vera Lehmensiek
    • 1
  • Gaby Ulrichs
    • 1
  • Ulrich Bilitewski
    • 2
  • Karsten Krohn
    • 2
  • Hartmut Höke
    • 3
  • Fritz Wagner
    • 1
  1. 1.Institute of Biochemistry and Biotechnology ATechnical University of BraunschweigBraunschweigFRG
  2. 2.Institute of Organic ChemistryTechnical University of BraunschweigBraunschweigFRG
  3. 3.Rütgers-BioTechMannheimFRG

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