Summary
A michaelian kinetic behaviour was found when the α-ramnosidase activity, both of native and immobilized hesperidinase, was determined on hesperidin suspensions. In spite of the low hesperidin solubility in the reaction medium, the maximum rates overtook the expected values, thus pointing to the enzyme ability to degrade insoluble hesperidin.
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Sánchez, M.A., Romero, C., Manjón, A. et al. Activity of soluble and immobilized hesperidinase on insoluble hesperidin. Biotechnol Lett 9, 871–874 (1987). https://doi.org/10.1007/BF01026201
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DOI: https://doi.org/10.1007/BF01026201