Summary
E. coli cells harbouring plasmid pBR322 which confers ampicillin resistance were immobilized on cotton cloth. The resulting film was used as an inoculum in daily repeated batch culture in ampicillin-free medium. During two months, the film was able to produce cultures which, at the late log phase, showed little sensitivity to 10 mg/ml ampicillin. Thus such a bacterial film can effectively be used as an inoculum for the production of recombinant DNA products by means of pBR322 or its derivatives in the absence of ampicillin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heyneker, H.L., Boyer, H.W., Crosa, J.H. and Falkow, S. (1977). Gene 2, 95–113.
Carrier, M.J., Nugent, M.E., Tacon, W.C.A. and Primrose, S.B. (1983). Trends in Biotechnology 1, 109–113.
Choma, C.T. and Yamazaki, H. (1981). Can. J. Microbiol. 27, 547–550.
Joshi, S. and Yamazaki, H. (1985). Biotechnology Letters 7, 753–758.
Joshi, S. and Yamazaki, H. (1987a). Biotechnology Letters 9, 43–46.
Joshi, S. and Yamazaki, H. (1987b). Biotechnology Techniques 1, 45–48.
Miller, J.H. (1972). In: Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, New York. 431–432.
Wouters, J.T.M., Driehuis, F.L., Polaczek, P.J., Van Oppenraay, M.-L.H.A. and vanAndel, J.G. (1980). Antonie van Leevwenhoek 46, 353–362.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Joshi, S., Yamazaki, H. Stability of plasmid PBR322 in Escherichia coli immobilized on cotton cloth during use as resident inoculum. Biotechnol Lett 9, 825–830 (1987). https://doi.org/10.1007/BF01026190
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01026190