Skip to main content
SpringerLink
Log in

Characterization of three distinct forms of lipolytic enzyme in a commercialCandida lipase preparation

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Three distinct forms of lipolytic enzyme were identified in a commercialCandida lipase preparation. Two of these lipases (lipases A & C) were isolated and characterized. Lipase A had a higher optimal reaction pH and a better thermal stability than those of lipase C. Lipase A and C displayed different acyl chain length specificity on the lipolysis of p-nitrophenol esters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Macrae, A. R., 1983, JAOCS,60, 243–246

    Google Scholar 

  2. Shaw, J. F. and Klibanov, A. M., 1987, Biotechnology & Bioengineering,29, 648–651

    Google Scholar 

  3. Shaw, J. F. and Liaw, E. T., 1987, in “Biocatalysis in Organic Media” ed. C. Lanne, J. Tramper and M. D. Lilly, 233–239, Elservier Science Publishers, Amsterdam

    Google Scholar 

  4. Shaw, J. F. and Chang, C. H., 1988. Bot. Bull. Acad. Sinica.29, 101–107

    Google Scholar 

  5. Chang, R. C. and Shaw, J. F., 1987, Bot. Bull. Acad. Sinica.,28, 33–42

    Google Scholar 

  6. Cambou, B and Klibanov, A.M., 1984, Biotech. & Bioeng.,26, 1449–1454

    Google Scholar 

  7. Veeraragavan, K. and Gibbs, B. F., 1989, Biotechnology Letters,11, 345–348

    Google Scholar 

  8. Hanes, B. D., 1981, in “Gel Electrophoresis of Proteins: A Practical Approach (ed. B. D. Hanes and D. Rickwood)”, p. 71, IRL Press Ltd., Oxford, Washington, D.C.

    Google Scholar 

  9. Tanksley, S. D. and Rick, C. M., 1980, Theor. Appl. Genet.,56, 209–219

    Google Scholar 

  10. Worthington Enzyme manual, Worthington Biochemical Co., Freehold, New Jersey, USA

  11. Amano Technical Bulletin for Lipase G Assay, Amano Pharmaceutical Co. Ltd., Nagoya, Japan

  12. Wang, Y.J., Sheu, J.Y., Wang, F.F. and Shaw, J.F., Biotech. & Bioeng., 1988,31, 628–633.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Botany, Academia Sinica, Taipei, Taiwan, ROC

    J. F. Shaw & C. H. Chang

  2. Institute Biomedical Engineering, National Yang Ming Medical College, Taipei, Taiwan, R O C

    Y. J. Wang

Authors
  1. J. F. Shaw
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. H. Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. J. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shaw, J.F., Chang, C.H. & Wang, Y.J. Characterization of three distinct forms of lipolytic enzyme in a commercialCandida lipase preparation. Biotechnol Lett 11, 779–784 (1989). https://doi.org/10.1007/BF01026096

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01026096

Keywords

Search

Navigation