Fructose production from Jerusalem artichoke by inulinase immobilized on chitin
- 115 Downloads
Inulinase fromAspergillus ficuum was immobilized by cross-linking with glutaraldehyde on chitin. Batch and continuous production of fructose from Jerusalem artichoke tuber was studied using this immobililized inulinase. In a batch reactor, the extent of hydrolysis attained 90% (D-fructose/D-glucose :86/14) in 10h and 77.5g/L of D-fructose was produced from the Jerusalem artichoke tuber juice. In a continuous packed bed column reactor, the maximum volumetric productivity of 61 g/L, h was obtained at residence time of 0.9h and conversion yield of 55%. At a fixed residence time of 2.6 h and 40° C, this could be operated for over two weeks with only a slight loss of activity (4.8%).
KeywordsFructose Chitin Batch Reactor Volumetric Productivity Conversion Yield
Unable to display preview. Download preview PDF.
- Bajpai, P. and Margaritis, A. (1985a) Enzyme Microb. Technol. 7, 373–376Google Scholar
- Bajpai, P. and Margaritis, A. (1985b). Enzyme Microb. Technol. 7, 459–461Google Scholar
- Bergmeyer, H. U. and Bert, E. Method of Enzymatic Analysis (Bergmeyer, H., ed), Verlag Chemie Weinheim, USA, 1974, Vol. 3, pp. 1205–1215)Google Scholar
- Byun, S. M. and Nahm, B. H. (1978) J. Food Sci. 43, 1871–1873Google Scholar
- Fleming, S. E. and GrootWassink, J. W. D. (1979) C. R. C. Crit. Rev. Food Sci. Nutr. 12, 1–28Google Scholar
- Guiraud, J. P., Demeulle, S. and Galzy, P. (1981) Biotechnol. Lett. 3, 683–688Google Scholar
- Kim, W. Y. and Byun, S. M. (1982) Enzyme Microb. Technol. 4, 239–244Google Scholar
- Miller, G. L. (1959). Analyt. Chem. 31, 426–428Google Scholar
- Toran-Diaz, I., Jain, V. K., Allais, J-J. and Baratti, J. (1985) Biotechnol. Lett. 7, 527–530Google Scholar
- Weiner, J. (1978) J. Inst. Brew. 88, 222–223Google Scholar
- Zittan, L. (1981) Starch 33, 373–377Google Scholar