Biotechnology Letters

, Volume 11, Issue 3, pp 201–206 | Cite as

Fructose production from Jerusalem artichoke by inulinase immobilized on chitin

  • C. H. Kim
  • S. K. Rhee
Article

Summary

Inulinase fromAspergillus ficuum was immobilized by cross-linking with glutaraldehyde on chitin. Batch and continuous production of fructose from Jerusalem artichoke tuber was studied using this immobililized inulinase. In a batch reactor, the extent of hydrolysis attained 90% (D-fructose/D-glucose :86/14) in 10h and 77.5g/L of D-fructose was produced from the Jerusalem artichoke tuber juice. In a continuous packed bed column reactor, the maximum volumetric productivity of 61 g/L, h was obtained at residence time of 0.9h and conversion yield of 55%. At a fixed residence time of 2.6 h and 40° C, this could be operated for over two weeks with only a slight loss of activity (4.8%).

Keywords

Fructose Chitin Batch Reactor Volumetric Productivity Conversion Yield 

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • C. H. Kim
    • 1
  • S. K. Rhee
    • 1
  1. 1.Biochemical Process Laboratory, Genetic Engineering CenterKorea Advanced Institute of Science and TechnologySeoulKorea

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