Summary
The effect of pH on growth and lactic acid production ofLactobacillus helveticus was investigated in a continuous culture using supplemented whey ultrafiltrate. Maximum lactate productivity of 5 gl−1h−1 occurred at pH 5.5. Whey permeates concentrated up to four times were fermented using batch cultures. Maximum lactic acid concentration of 95 gl−1 was attained, but residual sugars indicated a possible limitation in growth factors.
Similar content being viewed by others
Abbreviations
- D:
-
Dilution rate [h−1]
- X:
-
Biomass [gl−1]
- Glu:
-
Glucose consentration [gl−1]
- Gal:
-
Galactose consentration [gl−1]
- S:
-
Substrate, Lactose consentration [gl−1]
- P:
-
Product, Lactate consentration [gl−1]
- Yp/s :
-
Yield, defined as ΔP/ΔS [gg−1]
- ri :
-
Rate of synthesis or consumption of i [gl−1h−1]
References
“Bergey's Manual of Determinative Bacteriology”, 8th ed. (1974); Buchanan and Gibbons Co-Editors, The Williams & Wilkins Company (Baltimore)
Boyaval P., Corre C. and Terré S. (1987): “Continuous Lactic Acid Fermentation with Concentrated Product Recovery by Ultrafiltration and Electrodialysis”,Biotechnol. Letters,9, 207–212
Boyaval P., Corre C. and Terré S. (1988): “Production d'acide lactique à partir de perméat de lactosérum par fermentation continue en réacteur à membrane”,Le Lait,68, 65–84
Cox G.C. and MacBean R.D. (1977): “Lactic Acid Production by Lactobacillus bulgaricus in Supplemented Whey Ultrafiltrate”,Austr. J. Dairy Technol.,32, 19–22
Hanson T.P. and Tsao G.T. (1972): “Kinetic Studies of the Lactic Acid Fermentation in Batch and Continuous Cultures”,Biotechnol. Bioeng.,14, 233–252
Keller K. and Gerhardt P. (1975): “Continuous Lactic Acid Fermentation of Whey to Produce a Ruminant Feed Supplement High in Crude Protein”,Biotechnol. Bioeng.,17, 997–1018
Luedeking R. and Piret E.L. (1959): “A Kinetic Study of the Lactic Acid Fermentation. Batch Process at Controlled pH”,J. Biochem. Microbiol. Technol. Eng. 1, 393–412
Luedeking R. and Piret E.L. (1959): “Transient and Steady States in Continuous Fermentation. Theory and Experiment”,J. Biochem. Microbiol. Technol. Eng. 1, 431–459
Major N.C. and Bull A.T. (1985): “Lactic Acid Productivity of a Continuous Culture of Lb delbreuckii”,Biotechnol. Letters 7, 401–405
Mehaia M.A. and Cheryan M. (1986): “Lactic Acid from Acid Whey Permeate in a Membrane Recycle Bioreactor”,Enzyme Microb. Technol.,8, 289–292
Mehaia M.A. and Cheryan M. (1987): “Production of Lactic Acid from Sweet Whey Permeate Concentrates”,Process Biochem.,22, 185–188
Ohleyer E., Blanch H.W. and Wilke C.R. (1985): “Continuous Production of Lactic Acid in a Cell Recycle Reactor”,Appl. Biochem. Biotechnol.,11, 317–332
Ohleyer E., Wilke C.R. and Blanch H.W. (1985): “Continuous Production of Lactic Acid from Glucose and Lactose in a Cell Recycle Reactor”,Appl. Biochem. Biotechnol.,11, 457–463
Prigent Y. and Franco A. (1984): “Etude d'un fermenteur lactique à membranes”,Le Lait,64, 217–238
Reddy A.C., Henderson H.E. and Erdman M.D. (1976): Bacterial Fermentation of Cheese Whey for Production of a Ruminant Feed Supplement Rich in Crude Protein”,Appl. Env. Microbiol.,32, 769–776
Roy D., Goulet J. and LeDuy A. (1986): “Batch fermentation of Whey Ultrafiltrate by Lb helveticus for Lactic Acid Production”,Appl. Microbiol. Biotechnol.,24, 206–213
Roy D., LeDuy A. and Goulet J. (1987): “Kinetics of Growth and Lactic Acid Production from Whey Permeate by Lb helveticus”,Can. J. Chem. Eng.,65, 597–603
Stieber R.W. and Gerhardt P. (1979): “Continuous Process for Ammonium- Lactate Fermentation of Deproteinized Whey”,J. Dairy Sci.,62, 1558–1566
Stieber R.W. and Gerhardt P. (1981): “Dialysis Continuous Process for Ammonium Lactate Fermentation,”Biotechnol. Bioeng.,23, 523–549
Vahvaselkä M.I. and Linko P. (1987a): “Lactic Acid Fermentation in Milk Ultrafiltrate by Lb helveticus”,Proc. 4th Eur. Congress Biotechnol.,3, 317–320
Vahvaselkä M.I. and Linko P. (1987b): “Optimization of Lactic Acid Fermentation on Whey by Lactobacillus helveticus”,FEMS Microbiol. Reviews,46, 52
Vick Roy T.B., Mandel D.K., Dea D.K., Blanch H.W. and Wilke C.R. (1983): “The Application of Cell Recycle to Continuous Fermentatative Lactic Acid Production”,Biotechnol. Letters,5, 665–670
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aeschlimann, A., von Stockar, U. The production of lactic acid from whey permeate byLactobacillus helveticus . Biotechnol Lett 11, 195–200 (1989). https://doi.org/10.1007/BF01026058
Issue Date:
DOI: https://doi.org/10.1007/BF01026058