Summary
Cell-free extracts ofZymomonas mobilis were capable of fermenting glucose to ethanol and CO2 when stimulated by arsenate to act as an ATP uncoupler. 2M glucose was completely converted resulting in a final concentration of 16.5 % w/v ethanol. 1 M glucose was completely converted at temperatures up to 50°C. The results demonstrate that the glycolytic enzymes are more resistant to temperature and ethanol than are the living cells.
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Scopes, R.K., Griffiths-Smith, K. Fermentation capabilities ofZymomonas mobilis glycolytic enzymes. Biotechnol Lett 8, 653–656 (1986). https://doi.org/10.1007/BF01025976
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DOI: https://doi.org/10.1007/BF01025976