Biotechnology Letters

, Volume 8, Issue 9, pp 611–614 | Cite as

Production of active human interferon-β inE.coli II. Optimal condition for induced synthesis by 3,β-indoleacrylic acid

  • Tamio Mizukami
  • Tetsuo Oka
  • Seiga Itoh
Article

Summary

The maximum level of human interferon-β activity was expressed under the control of theE.coli tryptophan promoter whenE.coli cells were induced at late logarithmic growth phase by 3,β-indoleacrylic acid (IAA). The level is one order of magnitude higher than that obtained when the cells were induced at early logarithmic or stationary phase. When IAA was subsequently further added, the decrease in the activity observed at a latter period of fermentation was suppressed.

Keywords

Fermentation Organic Chemistry Optimal Condition Stationary Phase Tryptophan 

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References

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Copyright information

© Kluwer Academic Publishers 1986

Authors and Affiliations

  • Tamio Mizukami
    • 1
  • Tetsuo Oka
    • 1
  • Seiga Itoh
    • 1
  1. 1.Tokyo Research LaboratoriesKyowa Hakko Kogyo Co., Ltd.TokyoJapan

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