Summary
The maximum level of human interferon-β activity was expressed under the control of theE. coli tryptophan promoter whenE. coli cells were induced at late logarithmic growth phase by 3,β-indoleacrylic acid (IAA). The level is one order of magnitude higher than that obtained when the cells were induced at early logarithmic or stationary phase. When IAA was subsequently further added, the decrease in the activity observed at a latter period of fermentation was suppressed.
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Mizukami, T., Oka, T. & Itoh, S. Production of active human interferon-β inE. coli II. Optimal condition for induced synthesis by 3,β-indoleacrylic acid. Biotechnol Lett 8, 611–614 (1986). https://doi.org/10.1007/BF01025965
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DOI: https://doi.org/10.1007/BF01025965