Summary
A derivative ofEscherichia coli strain W3110 with increased tryptophan synthas (TS) activity was studied in the biosynthesis of L-tryptophan and 5-hydroxy-L-tryptophan, respectively, in presence of precursors. Indole or 5-hydroxyindole was added to growing cells in minimal medium supplemented with tetracycline. The specific activity of TS for 5-hydroxyindole was about 5-fold lower compared with indole. However, this difference in enzyme activity was not observed when the specific productivity (qp) of L-tryptophan or 5-hydroxy-L-tryptophan, which was 0.14–0.15 g (g dry wt cells)−1 · h−1 was determined. In minimal medium L-serine was shown to limit the production of both tryptophan and its hydroxylated derivative. In presence of L-serine, qp, for L-tryptophan and 5-hydroxy-L-tryptophan were increased by a factor of about 3 and 2, respectively.
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References
Daum, J. and Kieslich, K. (1974). Naturwissenschaften 61, 167–168.
Iriuchijima, S. and Tsuchiashi, G. (1978). Agr. Biol. Chem. 42, 843–845.
Lowry, O.H., N.J. Rosebrough, A.L. Farr and Randall, R.J. (1951). J Biol. Chem. 193, 265–275.
Miller, J.H. (1972). Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Nakazawa, H., Enei, H. and Okumura, S. (1972). Febrs. Letters 25, 43–45.
Skogman, G.S. and Sjöström, J.E. (1984). J. Gen. Microbiol. 130, 3091–3100.
Smith, D.H. and Yanofsky, C. (1962). Methods Enzymol. 5, 794–806.
Spies, J.R. and Chambers, D.C. (1948). Anal. Chem. 20, 30–39.
Wilcox, M. (1974) Anal. Biochem. 59, 436–440.
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Skogman, G.S., Sjöström, J.E. Biosynthesis of 5-hydroxy-L-tryptophan by a genetically modified strain ofEscherichia coli in presence of 5-hydroxyindole. Biotechnol Lett 7, 783–788 (1985). https://doi.org/10.1007/BF01025554
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DOI: https://doi.org/10.1007/BF01025554