Abstract
A new fluorescent reagent, Thiodan, containing the -SO2-S- function, is described. It can be utilized in protein chemistry as a reversible fluorescent labeling agent of cysteine residues. Thiodan is also shown to be useful for titration of sulfhydryl groups, due to the intensely fluorescent sulfinate anion released during the reaction.
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Caputo, R., Ferreri, C., Marino, G. et al. Synthesis, spectral properties, and use of Thiodan, a new thiol-specific fluorescent reagent. J Protein Chem 4, 133–140 (1985). https://doi.org/10.1007/BF01025373
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DOI: https://doi.org/10.1007/BF01025373