Journal of Protein Chemistry

, Volume 2, Issue 3, pp 251–261 | Cite as

Chemical and spectroscopic evidence on the homology of three antitumor proteins: α-sarcin, mitogillin, and restrictocin

  • José G. Gavilanes
  • David Vázquez
  • Fernando Soriano
  • Enrique Méndez
Article

Abstract

α-Sarcin, restrictocin, and mitogillin, three antitumor proteins, have been compared in terms of chemical composition as well as secondary and tertiary structure. The amino acid composition of the three polypeptides showed that restrictocin and mitogillin are essentially identical, α-Sarcin is also very similar to the other two proteins, although it lacks one methionine in its amino acid composition. Peptide maps of restrictocin and mitogillin coincide, except that one additional peptide is present in the mitogillin fingerprint. Although the α-sarcin map is different from the other two fingerprints, seven tryptic peptides with identical chromatographic and electrophoretic properties as well as amino acid composition were identified. The secondary and tertiary structures of mitogillin and restrictocin are identical from circular dichroism and difference spectroscopy studies. α-Sarcin has slightly different spectroscopic properties than the other two proteins. From these studies, the three proteins could be considered homologous polypeptides.

Key words

α-sarcin mitogillin restrictocin circular dichroism 

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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • José G. Gavilanes
    • 1
  • David Vázquez
    • 2
  • Fernando Soriano
    • 3
  • Enrique Méndez
    • 3
  1. 1.Departamento de Bioquímica, Facultad de CienciasUniversidad ComplutenseMadridSpain
  2. 2.Centro de Biología Molecular del CSIC y UAMMadridSpain
  3. 3.Servicio de EndocrinologíaCentro Ramón y CajalMadridSpain

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