Journal of Protein Chemistry

, Volume 2, Issue 3, pp 251–261 | Cite as

Chemical and spectroscopic evidence on the homology of three antitumor proteins: α-sarcin, mitogillin, and restrictocin

  • José G. Gavilanes
  • David Vázquez
  • Fernando Soriano
  • Enrique Méndez


α-Sarcin, restrictocin, and mitogillin, three antitumor proteins, have been compared in terms of chemical composition as well as secondary and tertiary structure. The amino acid composition of the three polypeptides showed that restrictocin and mitogillin are essentially identical, α-Sarcin is also very similar to the other two proteins, although it lacks one methionine in its amino acid composition. Peptide maps of restrictocin and mitogillin coincide, except that one additional peptide is present in the mitogillin fingerprint. Although the α-sarcin map is different from the other two fingerprints, seven tryptic peptides with identical chromatographic and electrophoretic properties as well as amino acid composition were identified. The secondary and tertiary structures of mitogillin and restrictocin are identical from circular dichroism and difference spectroscopy studies. α-Sarcin has slightly different spectroscopic properties than the other two proteins. From these studies, the three proteins could be considered homologous polypeptides.

Key words

α-sarcin mitogillin restrictocin circular dichroism 


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  1. Chen, Y. H., Yang, J. T., and Chau, K. H. (1974).Biochemistry 13, 3350–3359.Google Scholar
  2. Conde, F. P., Fernández-Puentes, C., Montero, M. T. V., and Vázquez, D. (1978).FEMS Microbiol. Lett. 4, 349–355.Google Scholar
  3. Ellman, G. L. (1959).Arch. Biochem. Biophys. 82, 70–77.Google Scholar
  4. Fernández-Puentes, C., and Carrasco, L. (1980).Cell 20, 769–775.Google Scholar
  5. Fernández-Puentes, C. and Vázquez, D. (1977).FEBS Lett. 78, 143–146.Google Scholar
  6. Matsubara, H., and Sasaki, R. M. (1969).Biochem. Biophys. Res. Commun. 35, 175–181.Google Scholar
  7. Méndez, E., and Lai, C. Y. (1975).Anal. Biochem. 65, 281–292.Google Scholar
  8. Olson, B. H. (1963). Alpha-sarcin, U.S. Patent 3, 104, 204.Google Scholar
  9. Olson, B. H., and Goerner, G. L. (1965).Appl. Microbiol. 13, 314–321.Google Scholar
  10. Olson, B. H., Jennings, J. C., Roga, V., Junek, A. J., and Schuurmans, D. M. (1965).Appl. Microbiol. 13, 322–326.Google Scholar
  11. Rodriguez, R., Lopez-Otin, C., Barber, D., Fernández-Luna, J. L., Gonzalez, G., and Méndez, E. (1982).Biochem. Biophys. Res. Commun. 108, 315–321.Google Scholar
  12. Roga, V., Hedeman, L. P., and Olson, B. H. (1971).Cancer Chemotherapy 55, 101–113.Google Scholar
  13. Schindler, D., and Davies, J. (1977).Nucleic Acid Res. 4, 1097–1110.Google Scholar

Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • José G. Gavilanes
    • 1
  • David Vázquez
    • 2
  • Fernando Soriano
    • 3
  • Enrique Méndez
    • 3
  1. 1.Departamento de Bioquímica, Facultad de CienciasUniversidad ComplutenseMadridSpain
  2. 2.Centro de Biología Molecular del CSIC y UAMMadridSpain
  3. 3.Servicio de EndocrinologíaCentro Ramón y CajalMadridSpain

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