Biotechnology Letters

, Volume 10, Issue 10, pp 713–718 | Cite as

Growth ofCatharanthusroseus cell suspensions in bioreactors: On-line analysis of oxygen and carbon dioxide levels in inlet and outlet gas streams

  • P. A. Bond
  • M. W. Fowler
  • A. H. Scragg


Catharanthusroseus cells (C87N) grown in a 30 litre airlift vessel achieved a growth rate of 0.366 day−1. The maximum biomass yield (9.13 gl−1) was recorded after 168 hours (7 days). On-line analysis of the composition of inlet and outlet gas streams during the growth cycle allowed calculation of the metabolic activity of the cultures. Oxygen uptake on a dry weight basis reached a maximum of 4.5×10−4 Moles O2 g dry weight−1 h−1 after 96 hours (during the mid-logarithmic phase of growth) and a maximum of 2.7×10−3 Moles O2 l−1 h−1 on a volume basis (towards the end of the logarithmic phase). Carbon dioxide production ran in parallel with oxygen use with maxima at 4.2×10−4 Moles CO2 g dry weight−1 h−1 and 3.4×10−3 Moles g l−1 h−1 respectively.


Biomass Carbon Dioxide Bioorganic Chemistry Oxygen Uptake Biomass Yield 
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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • P. A. Bond
    • 1
  • M. W. Fowler
    • 1
  • A. H. Scragg
    • 1
  1. 1.Wolfson Institute of Biotechnology The UniversitySheffieldU. K.

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