Biotechnology Letters

, Volume 11, Issue 9, pp 609–614 | Cite as

Continuous culture ofEscherichia coli for extracellular production of recombinant amylase

  • Peter Alexander
  • Patrick J. Oriel
  • David A. Glassner
  • Eric A. Grulke
Article

Summary

A recombinantEscherichia coli, grown in continuous culture, expressed aBacillus stearothermophilus α-amylase at 100-fold higher activities than theB. stearothermophilus itself. Excretion of the α-amylase to the supernatant was shown and found to be independent of the growth rate of the organism. Eleven to eighteen percent of the α-amylase was found in the supernatant. Dilution rates, or cell growth rates, ranging from 0.1 to 1.0 hours−1 were shown not to affect the compartmentation of the amylase and β-galactosidase.

Keywords

Growth Rate Cell Growth Organic Chemistry High Activity Amylase 

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Peter Alexander
    • 1
  • Patrick J. Oriel
    • 1
  • David A. Glassner
    • 2
  • Eric A. Grulke
    • 2
  1. 1.Department of Microbiology and Public HealthMichigan State UniversityEast Lansing
  2. 2.Department of Chemical EngineeringMichigan State UniversityEast Lansing

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