Journal of Protein Chemistry

, Volume 4, Issue 3, pp 185–197 | Cite as

Glutamine B16 insulin: Reduced insulin-like metabolic activity with moderately preserved mitogenic activity

  • Gerald P. Schwartz
  • Diana Wong
  • G. Thompson Burke
  • Monique A. de Vroede
  • Matthew M. Rechler
  • Panayotis G. Katsoyannis
Article

Abstract

An analogue of insulin in which the naturally occurring tyrosine residue in position B16 is replaced by a glutamine residue has been synthesized. Glutamine appears in the corresponding position in the B-domain of the insulin-like growth factors. This analogue displays 9% of the potency of insulin in binding to the insulin receptor from rat liver plasma membranes, 17% in stimulating the conversion of [3-3H] glucose into lipids in rat adipocytes, and 23% in insulin radioimmunoassay, but 40% of the potency of insulin in stimulating DNA synthesis in cultured chick fibroblasts. The analogue is a more potent mitogen than is a hybrid molecule which contains the A-chain of insulin and the entire B-domain sequence of IGF-I.

Key words

insulin analogue insulin-like growth factor metabolic assay mitogenic assay synthesis 

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Gerald P. Schwartz
    • 1
  • Diana Wong
    • 1
  • G. Thompson Burke
    • 1
  • Monique A. de Vroede
    • 2
  • Matthew M. Rechler
    • 2
  • Panayotis G. Katsoyannis
    • 1
  1. 1.Department of BiochemistryMount Sinai School of Medicine of the City University of New YorkNew York
  2. 2.Molecular, Cellular and Nutritional Endocrinology Branch, National Institute of Arthritis, Diabetes, and Digestive and Kidney DiseasesNational Institutes of HealthBethesda

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