Summary
2-Keto-3-deoxygluconate, an intermediate of glucose breakdown inSulfolobus solfataricus, was produced by enzymic dehydration of gluconate using whole cells of the micro-organism immobilized in crude egg white. The degradation of 2-keto-3-deoxygluconate to pyruvate and glyceraldehyde was avoided by inhibiting the aldolase activity in the cells by sodium borohydride treatment.
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References
De Rosa, M., Gambacorta, A., Nicolaus, B., Buonocore, V. and Poerio, E. (1980). Biotechnol. Lett. 2, 29–34.
De Rosa, M., Gambacorta, A., Nicolaus, B., Giardina, P., Poerio, E. and Buonocore, V. (1984). Biochem. J. 224, 407–414.
Grazi, E., Meloche, H., Martinez, G., Wood, W. A. and Horecker, B. L. (1963). Biochem. Biophys. Res. Comm., 10, 4–10.
Kornberg, A. (1955). Methods Enzymol. 1, 441–443.
Racker, E. (1957). Methods Enzymol. 3, 293–296.
Tomlinson, G. A., Koch, T. K. and Hochstein, L. I. (1974). Can. J. Microbiol. 20, 1085–1091.
Weissbach, A. and Hurwitz, J. (1959). J. Biol. Chem. 234, 705–709.
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Nicolaus, B., de Simone, A., del Pinao, L. et al. Production of 2-keto-3-deoxygluconate by immobilized cells of Sulfolobus solfataricus. Biotechnol Lett 8, 497–500 (1986). https://doi.org/10.1007/BF01025208
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DOI: https://doi.org/10.1007/BF01025208