Abstract
TPMP is a noncompetitive inhibitor of the nicotinic acetycholine receptor which blocks agonist-induced ion flux by directly interacting with the receptor's ion channel. By activation with UV light it can be made to react covalently with its binding site in the receptor protein. Here a method is described to perform this photolabeling with a time resolution comparable to the physiological event of channel opening and closing. By this technique structural transitions within the receptor can be visualized and transient conformational states can be labeled covalently and irreversibly.
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Hucho, F., Muhn, P. & Fahr, A. Photochemistry as a tool in investigating ionic channels: Photoaffinity probes. J Protein Chem 5, 99–107 (1986). https://doi.org/10.1007/BF01025194
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DOI: https://doi.org/10.1007/BF01025194