Journal of Protein Chemistry

, Volume 5, Issue 2, pp 99–107 | Cite as

Photochemistry as a tool in investigating ionic channels: Photoaffinity probes

  • Ferdinand Hucho
  • Peter Muhn
  • Alfred Fahr
Special Issue: The Fifth Symposium Of The Federation Of Asian And Oceanian Biochemists. “Enzyme Mechanisms” (Part I)


TPMP is a noncompetitive inhibitor of the nicotinic acetycholine receptor which blocks agonist-induced ion flux by directly interacting with the receptor's ion channel. By activation with UV light it can be made to react covalently with its binding site in the receptor protein. Here a method is described to perform this photolabeling with a time resolution comparable to the physiological event of channel opening and closing. By this technique structural transitions within the receptor can be visualized and transient conformational states can be labeled covalently and irreversibly.

Key words

acetylcholine receptor ion channel photoaffinity labeling TPMP, transient receptor states 


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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Ferdinand Hucho
    • 1
  • Peter Muhn
    • 1
  • Alfred Fahr
    • 2
  1. 1.Institut für Biochemie, Arbeitsgruppe NeurochemieFreie Universität BerlinBerlinWest Germany
  2. 2.Fachbereich Physik, Abt. BiophysikFreie Universität BerlinBerlinWest Germany

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