Abstract
The blood clamBarbatia virescens has a heterodimeric hemoglobin in erythrocytes. Interestingly, the congeneric clamsB. reeveana andB. lima contain quite different hemoglobins: tetramer and polymeric hemoglobin consisting of unusual didomain chain. The complete amino acid sequence of chain I ofB. virescens has been determined. The sequence was mainly determined from CNBr peptides and their subpeptides, and the alignment of the peptides was confirmed by sequencing of PCR-amplified cDNA forB. virescens chain I. The cDNA-derived amino acid sequence matched completely with the sequence proposed from protein sequencing.B. virescens chain I is composed of 156 amino acid residues, and the molecular mass was calculated to be 18,387 D, including a heme group. The sequence ofB. virescens chain I showed 35–42% sequence identity with those of the related clamAnadara trapezia and the congeneric clamB. reeveana. An evolutionary tree forAnadara andBarbatia chains clearly indicates that all of the chains are evolved from one ancestral globin gene, and that the divergence of chains has occurred in each clam after the speciation. The evolutionary rate for clam hemoglobins was estimated to be about four times faster than that of vertebrate hemoglobin. We suggest that blood clam hemoglobin is a physiologically less important molecule when compared with vertebrate hemoglobins, and so it evolved rapidly and resulted in a remarkable diversity in quaternary and subunit structure within a relatively short period.
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Chomczynski, P., and Sacchi, N. (1987).Anal. Biochem. 162, 156ia159.
Como, P. F., and Thompson, E. O. P. (1980).Aust. J. Biol. Sci. 33, 653–664.
Cox, L. R., Newell, N. D., and Boyd, D. W.,et al. (1969). InTreatise on Invertebrate Palaeontology, Part N, Vol. 2, Mollusca 6 Bivalvia (Moore, R. C., ed.), The Geol. Soc. Amer. and Univ. Kansas Press, Boulder, Colorado, pp. N248-N264.
Feng, D. F., Johnson, M. S., and Doolittle, R. F. (1985).J. Mol. Evol. 21, 112–125.
Fisher, W. K., Gilbert, A. T., and Thompson, E. O. P. (1984).Aust. J. Biol. Sci. 37, 191–203.
Gilbert, A. T., and Thompson, E. O. P. (1985).Aust. J. Biol. Sci. 38, 221–236.
Grinich, N. P., and Terwilliger, R. C. (1980).Biochem. J. 189, 1–8.
Naito, Y., Riggs, C. R., Vandergon, T. L., and Riggs, A. F. (1991).Proc. Natl. Acad. Sci. USA 88, 6672–676.
Riggs, C. R., and Riggs, A. F. (1991). InInvertebrate Dioxygen Carriers (Preaux, G., ed.), Leuven Univ. Press, Leuven, Belgium, pp. 57–60.
Royer, W. E., Love, W. E., and Fenderson, F. F. (1985).Nature 316, 277–280.
Suzuki, T. (1986).J. Biol. Chem. 261, 3692–3699.
Suzuki, T., Kapp, O. H., and Gotoh, T. (1988).J. Biol. Chem. 263, 18,524–18,529.
Suzuki, T., Shiba, M., Furukohri, T., and Kobayashi, M. (1989).Zool. Sci. 6, 269–281.
Terwilliger, R. C., and Terwilliger, N. B. (1985).Comp. Biochem. Physiol. 81B, 255–261.
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Suzuki, T., Nakamura, A., Satoh, Y. et al. Primary structure of chain I of the heterodimeric hemoglobin from the blood clamBarbatia virescens. J Protein Chem 11, 629–633 (1992). https://doi.org/10.1007/BF01024963
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DOI: https://doi.org/10.1007/BF01024963