Abstract
The primary structure of the 23S rRNA binding ribosomal protein L1 from the 50S ribosomal subunit ofThermus thermophilus ribosomes has been elucidated by direct protein sequencing of selected peptides prepared by enzymatic and chemical cleavage of the intact purified protein. The polypeptide chain contains 228 amino acids and has a calculated molecular mass of 24,694 D. A comparison with the primary structures of the corresponding proteins fromEscherichia coli andBacillus stearothermophilus reveals a sequence homology of 49% and 58%, respectively. With respect to both proteins, L1 fromT. thermophilus contains particularly less Ala, Lys, Gln, and Val, whereas its content of Glu, Gly, His, Ile, and Arg is higher. In addition, two fragments obtained by limited proteolysis of the intact, unmodified protein were characterized.
Similar content being viewed by others
References
Agalarov, S. Ch., Eliseikina, I. A., Sedelnikova, S. E., Fomenkova, N. P., Nikonov, S. V., and Garber, M. B. (1990).J. Mol. Biol. 216 501–502.
Baier, G., Piendl, W., Redl, B., and Stöffler, G. (1990).Nucl. Acids Res. 18 719–726.
Brauer, D., and Öchsner, I. (1978).FEBS Lett. 96 317–321.
Draper, D. E. (1989).Trends in Bioch. Sci. 14 335–338.
Garber, M. B., Agalarov, S. Ch., Eliseikina, I. A., Fomenkova, N. P., Nikonov, S. V., Sedelnikova, S. E., Shikaeva, O. S., Vasiliev, D., Zhdanov, A. S., Liljas, A., and Svensson, L. A. (1992).Biochimie 74 327–336.
Gogia, Z. V., Yusupov, M. M., and Spirina, T. N. (1986).Mol. Biol. (USSR) 20 519–526.
Gribskov, M., and Burgess, R. R. (1986).Nucl. Acids Res. 14 6745–6763.
Heinrich, T., Schröder, W., Erdmann, V. A., and Hartmann, R. K. (1992).J. Bacteriol. 174 7859–8763.
Itoh, T. (1988).Eur. J. Biochem. 176 297–301.
Jaenicke, R., and Závodsky, P. (1990).FEBS Lett. 268 344–349.
Jameson, B., and Wolf, H. (1988).Cabios 4 181–186.
Kimura, M., Kimura, J., and Ashman, K. (1985).Eur. J. Biochem. 150 491–497.
Littlechild, J., Malcolm, A., Paterakis, K., Ackermann, I., and Dijk, J. (1987).Biochim. Biophys. Acta 913 245–255.
Matheson, A. T., Auer, J., Ramírez, C., and Böck, A. (1990). InThe Ribosome, Structure, Function and Evolution (Hill, W. E.,et al., eds.), Am. Soc. Microbiol., Washington, pp. 617–635.
Menéndez-Arias, L., and Argos, P. (1989).J. Mol. Biol. 206 397–406.
Mrabet, N. T., Van den Broeck, A., Van den brande, I., Stanssens, P., Laroche, Y., Lambeir, A.-M., Matthijssens, G., Jenkins, J., Chiadmi, M., Van Tilbeurgh, H., Rey, F., Janin, J., Quax, W. J., Lasters, I., De Maeyer, M., and Wodak, S. J. (1992).Biochemistry 31 2239–2253.
Oakes, M. I., Scheinmann, A., Atha, T., Shankweiler, G., and Lake, J. A. (1990). InThe Ribosome, Structure, Function and Evolution (Hill, W. E.,et al., eds.), Am. Soc. Microbiol., Washington, pp. 180–193.
Oshima, T., and Imahori, K. (1974).Intern. J. System. Bacteriol. 24 102–112.
Schwartz, R. M., and Dayhoff, M. O. (1979). InAtlas of Protein Sequence and Structure (Dayhoff, M. O., ed.), Natl. Biomed. Res. Found., Washington, D.C., pp. 353–358.
Sedelnikova, S. E., Agalarov, S.Ch., Garber, M. B., and Yusupov, M. M. (1987).FEBS Lett. 220 227–230.
Shimmin, L. C., and Dennis, P. P. (1989).EMBO J. 8 1225–1235.
Sor, F., and Nomura, M. (1987).Mol. Gen. Genet. 210 52–59.
Stöffler-Meilicke, M., and Stöffler, G. (1990). InThe Ribosome, Structure, Function and Evolution (Hill, W. E.,et al., eds.), Am. Soc. Microbiol., Washington, pp. 123–133.
Trakhanov, S. D., Yusupov, M. M., Agalarov, S. C., Garber, M. B., Ryazantsev, S. N., Tishenko, S. V., and Shirokov, V. A. (1987).FEBS Lett. 220 319–322.
Villa, S., DeFazio, G., and Canosi, U. (1989).Anal. Biochem. 177 161–164.
Wrba, A., Schweiger, A., Schultes, V., and Jaenicke, R. (1990).Biochemistry 20 7584–7592.
Yonath, A., Bennett, W., Weinstein, S., and Wittmann, H. G. (1990). InThe Ribosome, Structure, Function and Evolution (Hill, W. E.,et al., eds.), Am. Soc. Microbiol., Washington, pp. 134–147.
Yusupova, G., Yusupov, M., Spirin, A., Ebel, J.-P., Moras, D., Ehresmann, C., and Ehresmann, B. (1991).FEBS Lett. 290 69–72.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Amons, R., Muranova, T.A., Rykunova, A.I. et al. The complete primary structure of ribosomal protein L1 fromThermus thermophilus. J Protein Chem 12, 725–734 (1993). https://doi.org/10.1007/BF01024930
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01024930