Abstract
A reliable high-performance liquid chromatography (HPLC) method is described for the separation of dimethylamino-azobenzenesulfonyl-amino acid (DABS-AA). The separation is accomplished by reversed-phase chromatography on a Hypersil ODS column (4.6×150 mm) withe a Hewlett-Packard liquid chromatography system. In addition to the developed sample and solvent preparation procedure, this precolumn modification method using dimethylaminoazobenzene sulfonyl chloride (DABS-CL) for amino acid analysis is proved reliable and sensitive. Five apolipoprotein B-100 tryptic peptides, two of them containing cysteine, were demonstrated as examples for the general application of this method in amino acid analysis. It is a useful method for analysis of cysteine- and cysteine-containing peptide and, furthermore, for determination of sulfhydryl and disulfide linkages of proteins.
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Yang, CY., Yang, T. & Yeh, B.K. Liquid chromatographic analysis of amino acids: Using dimethylamino-azobenzenesulfonyl chloride and hypersil ODS column to analyze the composition of apo B peptides. J Protein Chem 12, 11–14 (1993). https://doi.org/10.1007/BF01024908
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DOI: https://doi.org/10.1007/BF01024908