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Isolation of endopolygalacturonase hyperproducing mutants ofAspergillus sp. CH-Y-1043

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Summary

With the aim of obtaining hyperproducing strains of pectinases,Aspergillus sp. CH-Y-1043 was mutated with NTG and mutants resistant to glycerol catabolic repression were selected. Among the mutants obtained, CH-SS/ M63 produced an endo-PG activity 400% higher than the wild type, using lemon peel as the sole carbon source.

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References

  1. Fogarty, W.H. andWard, O.P. Progress in Industrial Microbiology.13, 59–119. (1974.

    Google Scholar 

  2. Fogarty, W.M. andKelly, C.T. Pectic enzymes. In:Microbial Enzymes and Biotechnology.W.M. Fogarty, Ed. 131–182, London.Applied Science Publishers (1983.

    Google Scholar 

  3. Angelova, M.B., Grigorov, I.V., Nikolova, N.S. and Bukano, R.D. Biologie Microbiol. 97–99. (1986).

  4. Shinmyo, A., Davis, I.K., Nomoto, F., Takara, T. andEnatsu, T. Eur. J. Appl. Microbiol. Biotechnol. 5, 59–68 (1978.

    Google Scholar 

  5. Keen, N.T. andHorton, J.C. Can. J. Microbiol. 12, 443–453 (1966.

    Google Scholar 

  6. Hsu, E.J. andVaughn, R.H. J. Bacteriol. 98, 172–181 (1969.

    Google Scholar 

  7. Shoemaker, S.P., Raymond, J.C. andBrener, C. Cellulases. In:Trends in the Biology of Fermentation for Fuel and Chemicals.Basic Life Science, N.Y. 89–109 (1981.

    Google Scholar 

  8. Montenecourt, B.S. and Eveleigh, D.E. In Hydrolysis of Cellulose Advan. Chem. Series. 14, 295–301 (1979).

  9. Montenecourt, B.S. andEveleigh, D.E. Appl. and Environm. Microbiol. 34, 777–782 (1977.

    Google Scholar 

  10. Gratzner, H. andSheehan, D.N. J. of Bacteriol. 97, 544–549 (1969.

    Google Scholar 

  11. Cuskey, E.M., Montenecourt, B.S. and Eveleigh, D.E. In: Overproduction of Microbial Products. 32, 405–408 (1981).

  12. Shelly, M.D., Frank, T.R. andWoods, A.R. J. of Gen. Microbiol. 132 893–898 (1986.

    Google Scholar 

  13. Durrands, P.K. andCooper, R.M. Appl. Microbiol. and Biotech. 28, 463–467 (1988.

    Google Scholar 

  14. Ilczuk, Z. Przemyst. Spoz., 9, 21–23 (1967.

    Google Scholar 

  15. Yoshiki, T., Akira, F. andHiroshi, N. J. Ferment. Technol. 66, 545–551 (1988.

    Google Scholar 

  16. Szajer, I. Acta Microbiol. Polon. 23, 119–123 (1974.

    Google Scholar 

  17. Szajer, I. Acta Microbiol. Polon. 5, 51–56 (1976.

    Google Scholar 

  18. Ilczuk, Z. Acta Microbiol. Polon. 25, 401–411 (1976.

    Google Scholar 

  19. Saval, S., Solórzano, R., Alpízar, L., Cea, A. and Huitrón, C. In: Biotecnologia de Enzimas. C. Huitrón, Ed. 203–215 (1983).

  20. Saval, S., Solórzano, R., Alpízar, L., Cea, A. and Huitrón, C. Proc. Int. Symp. on the Use of Enzymes in Food Technology. Versailles, France. 531–535 (1982).

  21. Huitrón, C., Saval, S. andAcuña, M.E. Ann. N.Y. Academ. Sciences 434, 110 (1984.

    Google Scholar 

  22. Larios, G., García, J.M. andHuitrón, C. Biotechnol. Lett. 11, 729–734 (1989.

    Google Scholar 

  23. Aguilar, G. andHuitrón, C. Enzyme Microb. Technol. 9, 541–545 (1986.

    Google Scholar 

  24. Aguilar, G. andHuitrón, C. Enzyme Microb. Technol. 9, 690–696 (1987.

    Google Scholar 

  25. Martinelli, S. andClutterbuck, A.J. J. of Gen. Microbiol. 69, 261–268 (1971.

    Google Scholar 

  26. Miller, L. Anal. Chem. 31, 426–428 (1959.

    Google Scholar 

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Author notes

  1. S. Solís

    Present address: Department of Biotechnology, UAM-Iztapalapa, 09340, México, D.F., México

Authors and Affiliations

  1. Department of Biotechnology. Institute of Biomedical Research, National University of Mexico UNAM, A.P. 70-228, 04510, Mexico, D.F., México

    S. Solís, M. E. Flores & C. Huitrón

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  1. S. Solís
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  2. M. E. Flores
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  3. C. Huitrón
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Solís, S., Flores, M.E. & Huitrón, C. Isolation of endopolygalacturonase hyperproducing mutants ofAspergillus sp. CH-Y-1043. Biotechnol Lett 12, 751–756 (1990). https://doi.org/10.1007/BF01024734

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