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Corrinoid production byMethanosarcina barkeri in a repeated fed-batch reactor with membrane module

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Summary

In an attempt to improve corrinoid production byM. barkeri strain Fusaro, a repeated fed-batch culture coupled with a membrane module on methanol-acetate medium was used. Productivity of 22 mg-corrinoid/1. day was obtained during 626 h cultivation with corrinoid and cell mass concentration of 95 mg/l and 25.9 g-dry cell/l, respectively. The minimum value for membrane flux permeation was 18 liter/m2. h for cell mass concentration between 25.9 to 31.0 g-dry cell/l. with rejection of 100 %.

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Authors and Affiliations

  1. Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo-cho, 724, Higashi-Hiroshima, Japan

    Roberto G. Silveira, Y. Nishida, N. Nishio & S. Nagai

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  1. Roberto G. Silveira
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  2. Y. Nishida
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  3. N. Nishio
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  4. S. Nagai
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Silveira, R.G., Nishida, Y., Nishio, N. et al. Corrinoid production byMethanosarcina barkeri in a repeated fed-batch reactor with membrane module. Biotechnol Lett 12, 721–726 (1990). https://doi.org/10.1007/BF01024728

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  • DOI: https://doi.org/10.1007/BF01024728

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