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Journal of Protein Chemistry

, Volume 10, Issue 1, pp 117–122 | Cite as

Irreversible inhibition of a monoclonal antibody by a nitrophenyl ester

  • Galla Rao
  • Manfred Philipp
Article

Abstract

TEPC-15 is a phosphorylcholine-binding mouse myeloma protein which reacts with an ester-containing phosphorylcholine, thep-nitrophenyl ester of 6-(phosphorylcholine)hexanoic acid (PEPCH). The rate of nitrophenolate release mediated by the antibody ispH-dependent and increases with increasingpH. The antibody becomes inactive during the reaction with the ester. The inactive antibody is not reactivated even after treatment with hydroxylamine. Antibody activity is associated with the Fab' fragment. These observations together with thepH profile of the reaction suggest that the ester acylates a lysine side chain near the antibody-binding site.

Key words

Abzyme affinity label antibody monoclonal antibody myeloma protein 6-(phosphorylcholine)hexanoic acid p-nitrophenyl-6-(phosphorylcholine)hexanoate 

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Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • Galla Rao
    • 1
  • Manfred Philipp
    • 1
  1. 1.Department of Chemistry, Lehman College/CUNY and Biochemistry Doctoral ProgramGraduate Center of the City University of New YorkBronx

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