Summary
Xylose isomerase was purified from a transformedE. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformedE. coli LE392 cells; no differences between these purified enzyme preparations were found.
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Tucker, M.Y., Tucker, M.P., Himmel, M.E. et al. Properties of genetically overproducedE. coli xylose isomerase. Biotechnol Lett 10, 79–84 (1988). https://doi.org/10.1007/BF01024630
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DOI: https://doi.org/10.1007/BF01024630