Abstract
In a previous study, we reported the GDP-dependent phosphorylation of a 36 kD membrane protein, p36, inD. discoideum membranes prepared from starved (aggregation competent) cells (Anschutzet al., 1989). Here we show that p36 can be phosphorylated when membranes are supplied either ATP or GTP as the phosphate donor, but that a greater level of p36 phosphorylation is achieved with GTP. The rate of phosphorylation of p36, using either nucleotide triphosphate, is enhanced by GDP. This reflects a decrease in the apparentK m of the enzyme for the particular nucleotide triphosphate. p36 can also be phosphorylated in membranes prepared from vegetative cells. However, the ability of GDP to stimulate p36 phosphorylation is not observed in vegetative cell membranes. Competition experiments indicate that there are also developmental differences in the nucleotide triphosphate site(s) available to phosphorylate p36.
Similar content being viewed by others
References
Anschutz, A., Howlett, A., and Klein, C. (1988).Proc. Natl. Acad. Sci. USA 86, 3665–3668.
Beebe, S. J., and Corbin, J. D. (1986). InThe Enzymes: Control by Phosphorylation (Boge, P. D., and Krebs, E. G., eds.), Academic, New York, Vol. 17, pp. 43–111.
Blackshear, P. J., Nairn, A. G., and Keeo, J. F. (1988).FASEB J. 14 2957–2969.
Edelman, A., Blumenthal, D., and Krebs, E. G. (1987).Annu. Rev. Biochem. 56, 567–613.
Ferguson, K. M., Hegashinima, T., Smigel, M. D., and Gilman, A. G. (1986).J. Biol. Chem. 261, 7393–7399.
Hunter, T. (1987).Cell 50, 823–829.
Juliani, M. H., Brusca, J., and Klein, C. (1981).Dev. Biol. 83, 114–121.
Juliani, M. H., and Klein, C. (1981).J. Biol. Chem. 256, 613–617.
Khachatrian, L., Howlett, A., and Klein, C. (1985).J. Cyc. Nuc. Prot. Phos. Res. 10, 179–188.
Khachatrian, L., Howlett, A., and Klein, C. (1987).Biochim. Biophys. Acta 927, 235–239.
Laemmli, U. K. (1970).Nature (London) 227, 680–685.
Lowry, O. M., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193, 265–275.
Meier, K., and Klein, C. (1988).Proc. Natl. Acad. Sci. USA,85, 2181–2185.
Neer, E. J., and Salter, R. S. (1981).J. Biol. Chem. 256, 12,102–12,107.
Shenolikar, S. (1987).J. Cyclic Nucleotide Protein Phosphorylation Res. 11, 531–541.
Shenolikar, S. (1988).FASEB J. 12, 2753–2764.
Sugimoto, Y., Erikson, E., Graziani, Y., and Erikson, R. L. (1985).J. Biol. Chem. 260, 13,838–13,843.
Sussman, M. (1966). InMethods in Cell Physiology (Prescott, B. N., ed.), Academic, New York, Vol. 2, pp. 397–410.
Taylor, S. S., and First, E. A. (1984).J. Biol. Chem. 259, 4011–4014.
Watts, D. J., and Ashworth, J. M. (1970).Biochem. J. 119, 171–174.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anschutz, A., Klein, C. Characterization of a GDP-sensitive phosphorylation in plasma membranes ofD. discoideum. J Protein Chem 9, 417–425 (1990). https://doi.org/10.1007/BF01024617
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01024617