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Journal of Protein Chemistry

, Volume 9, Issue 4, pp 389–395 | Cite as

An insulin-like hybrid consisting of a modified A-domain of human insulin-like growth factor I and the B-chain of insulin

  • Lin Zong
  • G. Thompson Burke
  • Panayotis G. Katsoyannis
Article

Abstract

We have synthesized an insulin-like compound, consisting of the B-chain of bovine insulin and an A-chain corresponding to the A-domain of human insulin-like growth factor-I (IGF-I), in which the isoleucine residue normally present in position 2 of the A-domain of IGF-I has been replaced with glycine. Biological evaluation of the compound indicated that its insulin-like activity (insulin receptor-binding and stimulation of lipogenesis) was 0.2%, and its growth-factor activity (stimulation of thymidine incorporation) was less than 1%, both relative to natural insulin. We conclude that interactions between IleA2 and TyrA19, which are crucial to high biological activity in insulin, are also present in IGF-I, and are equally critical for its biological activity.

Key words

IGF-I/insulin hybrid growth-promoting activity insulin activity structure-function relationships 

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Lin Zong
    • 1
  • G. Thompson Burke
    • 1
  • Panayotis G. Katsoyannis
    • 1
  1. 1.Department of BiochemistryMount Sinai School of Medicine of the City University of New YorkNew York

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