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Dissolved oxygen control of a maltose feed to batch fermentations ofStreptomyces clavuligerus: Effect on cephamycin C biosynthesis

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Summary

Compared to controls, a maltose-fed fermentation ofStreptomyces clavuligerus showed a 2-fold reduction in desacetoxycephalosporin C synthase activity and in the production of the antibiotic, cephamycin C. Accumulation of the pathway intermediate, penicillin N occurred in the control fermentations but not in the maltose-fed culture, indicating that the carbon source was also regulating steps earlier in the pathway.

Since the dissolved oxygen concentration was effectively maintained at almost constant levels in both the controls and maltose-fed fermentations, the observed maltose interference with cephamycin C biosynthesis was not related to the aeration condition of the actively growingS. clavuligerus culture.

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Authors and Affiliations

  1. Department of Microbiology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada

    M. J. Rollins, S. E. Jensen & D. W. S. Westlake

  2. Department of Chemistry, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    S. Wolfe

Authors
  1. M. J. Rollins
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  2. S. E. Jensen
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  3. S. Wolfe
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  4. D. W. S. Westlake
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Rollins, M.J., Jensen, S.E., Wolfe, S. et al. Dissolved oxygen control of a maltose feed to batch fermentations ofStreptomyces clavuligerus: Effect on cephamycin C biosynthesis. Biotechnol Lett 10, 295–300 (1988). https://doi.org/10.1007/BF01024423

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  • DOI: https://doi.org/10.1007/BF01024423

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