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Biotechnology Letters

, Volume 10, Issue 4, pp 273–276 | Cite as

The process of continuous production and isolation of coproporphyrin

  • Petr Kotal
  • Jan Sperl
  • Milan Jirsa
  • Vaclav Kordac
Article
  • 14 Downloads

Summary

The production of coproporphyrin from glucose and urea has been achieved by semi-aerobic culture ofSaccharomyces cerevisiae. The cells were entrapped in alginate gel and packed into a column reactor for continuous long-term process. The porphyrins were isolated and simultaneously concentrated from the effluent by liquid-solid phase extraction, purified by liquid chromatography and finally crystallized. Using the process described the final product of high purity was obtained.

Keywords

Glucose Chromatography Urea Organic Chemistry Liquid Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Petr Kotal
    • 1
  • Jan Sperl
    • 1
  • Milan Jirsa
    • 1
  • Vaclav Kordac
    • 1
  1. 1.Ist Department of MedicineCharles UniversityPrague 2Czechoslovakia

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