The process of continuous production and isolation of coproporphyrin
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The production of coproporphyrin from glucose and urea has been achieved by semi-aerobic culture ofSaccharomyces cerevisiae. The cells were entrapped in alginate gel and packed into a column reactor for continuous long-term process. The porphyrins were isolated and simultaneously concentrated from the effluent by liquid-solid phase extraction, purified by liquid chromatography and finally crystallized. Using the process described the final product of high purity was obtained.
KeywordsGlucose Chromatography Urea Organic Chemistry Liquid Chromatography
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