Summary
The effect ofin situ extraction and elicitor treatment on shikonin production was studied with the suspension cultures ofLithospermum erythrorhizon. Shikonin concentration of 60 mg/L was achieved by the use of both techniques which was 24 times higher than that of control culture, and 65 times higher in terms of shikonin productivity. The host-pathogen effect of elicitor treatment andin situ extraction for product removal were effective for shikonin production.
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Kim, D.J., Chang, H.N. Increased shikonin production inLithospermum erythrorhizon suspension cultures within situ extraction and fungal cell treatment (elicitor). Biotechnol Lett 12, 443–446 (1990). https://doi.org/10.1007/BF01024401
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DOI: https://doi.org/10.1007/BF01024401