Abstract
Analyses of two-dimensional chromatographic flavonoid patterns of butterflies reared on different grass species have shown that the flavonoid pattern ofMelanargia galathea is dependent on the flavonoid content of the larval diet. This confirms the dietary origin of flavonoid pigments inM. galathea. The flavonoid patterns of butterflies reared on different grass species differ from each other and from the larval food plants;M. galathea reared on the same grass species have identical flavonoid patterns. Differences in the butterfly and larval food plant flavonoid patterns indicate that the ingested flavonoids are metabolized byM. galathea or its gut flora before sequestration. The distinct flavonoid patterns of butterflies reared on different larval food plants have been defined as the flavonoid fingerprint profiles for each grass species. Similarity between theFestuca rubra flavonoid fingerprint profile and the constant flavonoid pattern characteristic of wild-capturedMelanargia suggests thatMelanargia larvae are not generalist grass feeders, but are specific toF. rubra or toF. rubra and a few closely related grass species in the wild.
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Aplin, R.T., andRothschild, M. 1972. Poisonous alkaloids in the body tissues of the garden tiger moth (Arctia caja L.) and the cinnabar moth (Tyria (Callimorpha) jacobaeae L.) (Lepidoptera), pp. 579–595,in A. de Vries and E. Kochra (eds.). Toxins of Plant and Animal Origin. Gordon and Breach, London.
Brower, L. 1969. Ecological chemistry.Sci. Am. 220:22–29.
Brower, L.P., Seiber, J.N., Nelson, C.J., Lynch, S.P., andTuskes, P.M. 1982. Plant-determined variation in the cardenolide content, thin-layer chromatographic profiles, and emetic potency of monarch butterflies,Danaus plexippus reared on the milkweed,Asclepias eriocarpa in California.J. Chem. Ecol. 8:579–633.
Duffey, S.S., andSchudder, G.G.E. 1974. Cardiac glycosides inOncopeltus fasciatus (Dallas) (Hemiptera: Lygaeidae). I. The uptake and distribution of natural cardenolides in the body.Can. J. Zool. 52:283–290.
Edgar, J.A., andCulvenor, C.C.J. 1974. Pyrrolizidine ester alkaloid in danaid butterflies.Nature 250:614–616.
Edgar, J.A., Culvenor, C.C.J., andPliske, T.E. 1974. Coevolution of daniad butterflies with their host plants.Nature 250:646–648.
Feltwell, J.S., andValadon, L.R.G. 1970. Plant pigments identified in the common blue butterfly.Nature 255:969.
Ford, E.B. 1941. Studies on the chemistry of pigments in the lepidoptera, with reference to their bearing on systematics. 1. The anthoxanthins.Proc. R. Entomol. Soc. London. (A) 16:65–90.
Ford, E.B. 1944. Studies on the chemistry of pigments in the Lepidoptera, with reference to their bearing on systematics. 4. The classification of the Papilionidae.Trans. R. Entomol. Soc. London (A) 19:92–106.
Fujimoto, N., Hayashiya, K., andJono, S. 1959. Studies on the pigments of cocoon (V) On the pigments of cocoon inTheophila mandarina Moore.J. Sericult. Sci. Jpn. 28:84–87.
Harashima, K., Ohno, T., Sawachika, T., Hidaka, T., andOhnishi, E. 1972. Carotenoids in orange pupae of the swallowtail,Papilio xuthus.Insect Biochem. 2:29–48.
Harashima, K., Nakahara, J., andKato, G. 1976. Papilioerythinone: A new ketocarotenoid in integuments of orange pupae of a swallowtailPapilio xuthus, and carapaces of a crab,Paralithodes brevipes.Biol. Chem. 40:711–717.
Harborne, J.B. 1967. Comparative Biochemistry of the Flavonoids. Academic Press, London.
Harborne, J.B. 1973. Phytochemical Methods. Chapman and Hall, London.
Harborne, J.B., andMabry, T.J. 1982. The Flavonoids: Advances in Research. Chapman and Hall, London.
Harborne, J.B., Mabry, T.J., andMabry, H. 1975. The Flavonoids. Academic Press, New York.
Hayashiya, K., Sugimoto, S., andFujimoto, N. 1959. Studies on the pigments of cocoon. (III) The qualitative test of the pigments of green cocoon.J. Sericult. Sci. Jpn. 28:27–32.
Hubbard, C.E. 1968. Grasses. Penguin Books Ltd., Middlesex.
Kayser, H. 1982. Carotenoids in insects, pp. 195–210,in G. Britton and T. W. Goodwin (eds.). Carotenoid Chemistry and Biochemistry. Pergamon Press, Oxford.
Morris, S.J., andThomson, R.H. 1963. The flavonoid pigments of the marbled white butterfly (Melanargia galathea Seltz).J. Insect Physiol. 9:391–399.
Morris, S.J., andThomson, R.H. 1964. The flavonoid pigments of the small heath butterfly,Coenonympha pamphilus L.J. Insect Physiol. 10:377–383.
Reichstein, T. von, Euw, J., Parsons, J.A., andRothschild, M. 1968. Heart poisons in the monarch butterfly.Science 161:861–866.
Roeske, C.N., Seiber, J.S., Brower, L.P., andMoffitt, C.M. 1976. Milkweed cardenolides and their processing by monarch butterflies (Danaus plexippus L.).Recent. Adv. Phytochem. 10:93–167.
Thomson, D.L. 1926. The pigments of butterflies' wings. I.Melanargia galathea.Biochem. J. 20:73–75.
Wilson, A. 1985. Flavonoid pigments in the genusMelanargia. Phytochemistry In press.
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Wilson, A. Flavonoid pigments in marbled white butterfly (Melanargia galathea) are dependent on flavonoid content of larval diet. J Chem Ecol 11, 1161–1179 (1985). https://doi.org/10.1007/BF01024106
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DOI: https://doi.org/10.1007/BF01024106