Summary
Phenol degradation by free and immobilized cells ofFusarium flocciferum was studied in a chemostat at steady-state conditions. For the free cell system the dilution rates varied from 0.02 to 0.13h−1, with a total phenol removal up to 0.08h−1. Wash-out seemed to set in at 0.11h−1. The immobilized cells showed virtually complete phenol utilization at 1g/l, over a period of four months. At D=0.2h−1 and above 1g/l phenol, the complete phenol removal is not achieved: a progressive increase in the outlet concentration was observed attaining a value of 284mg/l at 1.5g/l.
Similar content being viewed by others
References
Anselmo, A.M., Novais, J.M. (1984).Biotechnol. Letters 6, 601–606.
Atlow, S.C., Bonadonna-Aparo, L. Klibanov, A.M. (1984).Biotechnol. Bioenng. 26, 599–603.
Davis, S., Burns, R.G. (1990).Appl. Microbiol. Biotechnol. 32, 721–726.
Holladay, D.W., Hancher, C.W., Scott, C.D. (1978).J. Water Pollution Control Fed. 50, 2573–2589.
Lewandowski, G.A., Armenante, P.M., Pak, D. (1990).Water Res., 24, 75–82.
Lin, J-Er., Wang, H.Y., Hickey, R.F. (1990).Biotechnol. Bioeng. 35, 1125–1134.
Livernoche, D., Jurasek, L., Desrochers, M., Dorica, J. (1984).Biotechnol. Bioeng., 25, 2055–2065.
Macris, B.J., Kokke (1978).Biotechnol. Bioeng., 20, 1027–1035.
Melling, J. (1977). Regulation of enzyme synthesis in continuous culture. InTopics in Enzyme and Fermentation Biotechnology, Vol. 1, Chap. 2, pp. 10–42. Ed. Alan Wiseman, Ellis Horwood Ltd., Chichester.
Nazly, N., Knowless, C.J., Beardsmore, A.J., Naylor, W.T., Corcoran, E.G. (1983).J. Chem. Techn. Biotechnol. 33B, 119–126.
Righelato, R.C. (1975). Growth kinetics of mycelial fungi. InFilamentous Fungi, Vol. 1, Chap. 5, pp. 790–103. Ed. J. E. Smith and D. B. Berry, Edward Arnold (Publishers) Limited, London.
Royer, G., Desrochers, M. Jurasek, L., Rouleau, D., Mayer, R.C. (1985).J. Chem. Techn. Biotechnol. 35B, 14–22.
Silva, M.E.S.T., Nicoli, J.R. (1985).J. Ferment. Technol., 63, 91–94.
Wisecarver, K.D., Fan, L.-S. (1989).Biotechnol. Bioeng, 33, 1029–1038.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anselmo, A.M., Novais, J.M. Biological treatment of phenolic wastes: Comparison between free and immobilized cell systems. Biotechnol Lett 14, 239–244 (1992). https://doi.org/10.1007/BF01023366
Issue Date:
DOI: https://doi.org/10.1007/BF01023366