Summary
A process was developed for the production ofTrigonopsis variabilis and D-amino acid oxidase. Yields for the yeast were in excess of 220 g/l wet weight and 62 g/l dry weight. Using cephalosporin C as a substrate the enzyme concentration was 7000 units per liter.
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Huber, F.M., Vicenzi, J.T. & Tietz, A.J. High yielding culture conditions for the biosynthesis of D-amino acid oxidase byTrigonopsis variabilis . Biotechnol Lett 14, 195–200 (1992). https://doi.org/10.1007/BF01023358
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DOI: https://doi.org/10.1007/BF01023358