Abstract
Enzymatic deacidification reaction of hyperacid oils, biocatalyzed by a lipase ofMucor miehei (Lipozyme), was optimized on a reconstituted acid oil, according to an experimental plan with the following variable parameters: pressure, temperature, proportion of biocatalyst. The optimum values defined (20 mm Hg, 60°C, 5.5% w/w of Lipozyme) allowed to determine reaction kinetics and to obtain a yield of 93%.
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This work was carried out under a contract from the Conseil Général de l'Hérault (no 91.1108).
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Ducret, A., Pina, M., Montet, D. et al. Biocatalysed deacidification: Reaction optimization on a model hyperacid oil. Biotechnol Lett 14, 185–188 (1992). https://doi.org/10.1007/BF01023356
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DOI: https://doi.org/10.1007/BF01023356