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Stability performance ofCynara cardunculus L. acid protease in aqueous-organic biphasic systems

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Abstract

Stability performance of the acid protease ofCynara cardunculus L. in biphasic systems containing ethyl acetate,n-hexane or isooctane was investigated and compared with that of pepsin. Activity retention was higher in the system containingn-hexane. In this system 100% retention was observed up to 144 hours. Pre-saturation of phases was found to increase enzyme stability in the cases ofn-hexane and isooctane and to be an absolute requirement in the case of ethyl acetate. The results obtained suggest also that, when dealing with pre-saturated phases, log P cannot be used straightforwardly to predict enzyme stability in biphasic systems.

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Authors and Affiliations

  1. Centro de Biologia Celular, Universidade de Coimbra, 3000, COIMBRA, Portugal

    M. T. Barros & E. M. V. Pires

  2. Departamento de Engenharia Química, Universidade de Coimbra, 3000, COIMBRA, Portugal

    M. G. V. Carvalho & F. A. P. Garcia

Authors
  1. M. T. Barros
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  2. M. G. V. Carvalho
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  3. F. A. P. Garcia
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  4. E. M. V. Pires
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Barros, M.T., Carvalho, M.G.V., Garcia, F.A.P. et al. Stability performance ofCynara cardunculus L. acid protease in aqueous-organic biphasic systems. Biotechnol Lett 14, 179–184 (1992). https://doi.org/10.1007/BF01023355

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