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Biotechnology Letters

, Volume 14, Issue 3, pp 179–184 | Cite as

Stability performance ofCynara cardunculus L. acid protease in aqueous-organic biphasic systems

  • M. T. Barros
  • M. G. V. Carvalho
  • F. A. P. Garcia
  • E. M. V. Pires
Article

Abstract

Stability performance of the acid protease ofCynara cardunculus L. in biphasic systems containing ethyl acetate,n-hexane or isooctane was investigated and compared with that of pepsin. Activity retention was higher in the system containingn-hexane. In this system 100% retention was observed up to 144 hours. Pre-saturation of phases was found to increase enzyme stability in the cases ofn-hexane and isooctane and to be an absolute requirement in the case of ethyl acetate. The results obtained suggest also that, when dealing with pre-saturated phases, log P cannot be used straightforwardly to predict enzyme stability in biphasic systems.

Keywords

Enzyme Acetate Ethyl Organic Chemistry Ethyl Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • M. T. Barros
    • 1
  • M. G. V. Carvalho
    • 2
  • F. A. P. Garcia
    • 2
  • E. M. V. Pires
    • 1
  1. 1.Centro de Biologia CelularUniversidade de CoimbraCOIMBRAPortugal
  2. 2.Departamento de Engenharia QuímicaUniversidade de CoimbraCOIMBRAPortugal

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