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Simultaneous fructose and ethanol production from sucrose, usingZymomonas mobilis 2864 co-immobilised with invertase

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Summary

Fructokinase negativeZymomonas mobilis UQM 2864, was co-immobilised with invertase in alginate and incubated on sucrose-based media in batch and fedbatch culture. The highest fructose concentration achieved was 138 g/l using fed-batch cultivation with sugar-cane syrup-simultaneously producing 79.9 g/l or 10.1% (v/v) ethanol in less than 24 hours. The ethanol and fructose yields were 95 and 84% respectively. Co-immobilisation resulted in faster fermentation times, particularly for the batch fermentations, and complete utilisation of substrate.

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Authors and Affiliations

  1. Microbiology Department, The University of Queensland, 4072, Brisbane, Queensland, Australia

    Lisa A. Kirk

  2. MIRCEN-brisbane, The University of Queensland, 4072, Brisbane, Queensland, Australia

    Horst W. Doelle

Authors
  1. Lisa A. Kirk
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  2. Horst W. Doelle
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Kirk, L.A., Doelle, H.W. Simultaneous fructose and ethanol production from sucrose, usingZymomonas mobilis 2864 co-immobilised with invertase. Biotechnol Lett 16, 533–538 (1994). https://doi.org/10.1007/BF01023339

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