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High production of xanthan gum by a strain ofXanthomonas campestris conjugated withLactococcus lactis

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Summary

Plasmid pNZ521, containing phospho-β-galactosidase, maturation protein and proteinase P genes, was conjugally transferred for the first time fromL.lactis intoX.campestris XLM1.After 20 generations 67% of the tested colonies were resistant to chloramphenicol. In the transconjugant proteinase activity appeared in the growth medium, whereas inL.lactis MG1820 it was extracted from the cell wall. Proteinase activity and production of xanthan gum were studied in different concentrations of whey. Xanthan gum production was found much higher in all cultures with the transconjugant strain XLM1521.

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Ekateriniadou, L.V., Papoutsopoulou, S.V. & Kyriakidis, D.A. High production of xanthan gum by a strain ofXanthomonas campestris conjugated withLactococcus lactis . Biotechnol Lett 16, 517–522 (1994). https://doi.org/10.1007/BF01023336

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