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Cloning theClostridium thermocellum thermostable laminarinase gene inEscherichia coli: The properties of the enzyme thus produced

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Summary

We have cloned a 1.9-kb-long fragment ofClostridium thermocellum DNA which encodes laminarinase (EC 3.2.1.39). The enzyme hydrolyzes the β-1,3-glucoside bonds in β-1,3-and in mixed β-1,3-1,4-polyglucans. The enzyme's optimum pH value is around 8.5, temperature optimum −70°C. PAGE-determined mol. weight −32 kDa.

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Abbreviations

CMC:

carboxymethyl cellulose

pNPC:

p-nitrophenyl β D cellobioside

pNPLac:

p-nitrophenyl-β D-lactoside

pNPG:

p-nitrophenyl β D glucopyranoside

pNPGal:

p-nitrophenyl-β D galactopyranoside

pNPXyl:

p-nitrophenyl-β

D:

xylopyranoside

Ap:

ampicillin

SDS-PAGE:

SDS polyacrylamide gel electrophoresis

References

  • Beguin, P. (1982). Anal. Biochem. 131, 333–336.

    Google Scholar 

  • Bradford, M.M. (1976). Anal. Biochem. 72, 248.

    Google Scholar 

  • Bumazkin, B.K. Velikodvorskaja, G.A., Tuka, K., Mogutov, M.A., Strongin, A.Ya. (1990). Biochem. Biophys. Research Comm. 167 1057–1064

    Google Scholar 

  • Anari, T.M. and Machkanen, P.H. (1975). Proc. Am. Soc. Brew Chem. 33, 13–17.

    Google Scholar 

  • Hamlyn, P.F., Bradshaw, R.R., Mellon, F.M., Sanliago, C.M., Wilson, J.M. and Peberdy, J.F. (1981). Enzyme Microb. Technol. 3, 321–325.

    Google Scholar 

  • Hazlewood, G.P., Romaniec, M.P.M., Davidson, K., Grepinet, O., Beguin, P., Millet, J., Raynaud, O. and Aubert, J.-P. (1988). FEMS Microbiol. Lett. 51, 231–236.

    Google Scholar 

  • Laemmli, U.K. (1970). Nature 227, 680–685.

    Google Scholar 

  • Maniatis, T., Fritsch, E.F., Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.

    Google Scholar 

  • Miller, G.L., Blum, R., Glennon, W.E., Burton, A.L. (1960) Anal. Biochem. 2, 127–132.

    Google Scholar 

  • Schwarz, W., Bronnenmeier, K. and Staudenbauer, W.L. (1985). Biotechnol. Lett. 7, 859–864.

    Google Scholar 

  • Schwarz, W.H., Schimming, S. and Staudenbauer, W.L. (1988a) Biotechnol. Lett. 10, 225–230.

    Google Scholar 

  • Schwarz, W.H., Schimming, S. and Staudenbauer, W.L. (1988b). Appl. Microbiol. Biotechnol. 29, 25–31

    Google Scholar 

  • Sharma, A., and Nakas, J.P. (1987). Enzyme Microb. Technol. 9, 89–93.

    Google Scholar 

  • Teather, R.M. and Wood, P.J. (1982). Appl. Environ. Microbiol. 43, 777–780.

    Google Scholar 

  • Tuka, K., Zverlov, V.V., Bumazkin, B.K., Velikodvorskaya, G.A. and Strongin, A.Ya. (1990). Biochem. Biophys. Research Comm. (in press)

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Authors and Affiliations

  1. Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov sq. 16, 123182, Moscow, USSR

    V. V. Zverlov & G. A. Velikodvorskaya

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  1. V. V. Zverlov
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  2. G. A. Velikodvorskaya
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Zverlov, V.V., Velikodvorskaya, G.A. Cloning theClostridium thermocellum thermostable laminarinase gene inEscherichia coli: The properties of the enzyme thus produced. Biotechnol Lett 12, 811–816 (1990). https://doi.org/10.1007/BF01022600

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