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Isolation of the tyrosine phenol lyase gene from Citrobacter freundii

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Summary

The gene for the enzyme tyrosine phenol-lyase (TPL) was initially isolated on a 45 kbp fragment of Citrobacter freundii genomic DNA contained in a cosmid. Subsequent restriction enzyme digestion and sub-cloning resulted in the gene being contained on a 2.4 kbp DNA fragment.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, University of Strathclyde, The Todd Centre, 31 Taylor Street, G4 0NR, Glasgow, UK

    Henry Brzeski

  2. Institute of Technical Biochemistry, Technical University of Lodz, Stefanowskiego 4/10, 90-924, Lodz, Poland

    Jacek Polak

Authors
  1. Jacek Polak
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  2. Henry Brzeski
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Polak, J., Brzeski, H. Isolation of the tyrosine phenol lyase gene from Citrobacter freundii. Biotechnol Lett 12, 805–810 (1990). https://doi.org/10.1007/BF01022599

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