Summary
The influence of temperature, agitation speed, pH and biomass on the synthesis of 19 metabolites contributing to a strawberry aroma was followed over a 72 h fermentation of skim milk byPseudomonas fragi. Amongst the major odor-active metabolites were ethyl butyrate, ethyl hexanoate, ethyl 2-hexenoate, ethyl crotonate, ethyl isovalerate and ethyl 2-methyl hexanoate. Up to 17 ppm of some of these metabolites were detected at 60 h of fermentation, approximately 36 h after the beginning of the stationary growth phase. The production of most odor-active metabolites was higher at 11°C and 150 RPM than at 15, 20 or 25°C and 200 or 250 RPM. The development of off-aromas was observed at high temperatures and at high agitation speeds.
Similar content being viewed by others
Literature cited
Albretcht, J.J. (1987). Food Technol.,41:66–71.
Armstrong, J.J., Gillies, B. and Yamazaki, H. (1989).In: Flavor Chemistry, Trends and Developments. R. Teranishi, R.G. Buttery and F. Shahidi, eds. American Chemical Society, Symposium Series 388, pp. 105–120, Washington D.C.
Armstrong, D.W. and Yamazaki, M. (1986). Trends in Biotechnol.,4:264–268.
Bassette, R., Fung, D.Y.C. and Mantha, V.R. (1986). CRC Critical Reviews in Food Science and Nutrition,24(1):1–52.
Cormier, F., Raymond, Y., Champagne, C.P. and Morin, A. (1991). J. Agric. Food Chem. (in press, January)
Hosono, A. and Elliott, J.A. (1974). J. Dairy Sci.,57:1432–1437.
Hosono, A., Elliott, J.A. and McGugan, W.A. (1974). J. Dairy Sci.,57:535–539.
Latrasse, A., Degorge-Dumas, J.R. and Leveau, J.Y. (1985). Sci. des Aliments.5:1–26.
Nashif, S.A., and Nelson, F.E. (1953). J. Dairy Sci.,36:471–480.
Pereira, J.N. and Morgan, M.E. (1958). J. Dairy Sci.,41:1201–1205.
Reddy, M.C., Bills, D.D., Lindsay, R.C., Libbey, L.M. (1968), J. Dairy Sci.,51: 656–659.
Reddy, M.C., Bills, D.D., and Lindsay, R.C. (1969a). Appl. Microbiol.17:779–782.
Reddy, M.C., Lindsay, R.C. and Bills, D.D. (1969b). J. Dairy Sci.,52:1198–1201.
Sharpf, L.G., Seitz, E.W., Morris, J.A. and Farbood, M.I. (1986).In: Biogeneration of Aromas. T.H. Parliment, and R. Croteau, eds. American Chemical Society, Symposium Series 317, pp. 323–346, Washington D.C.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raymond, Y., Morin, A., Cormier, F. et al. Physical factors influencing the production of strawberry aroma byPseudomonas fragi grown in skim milk. Biotechnol Lett 12, 931–936 (1990). https://doi.org/10.1007/BF01022593
Issue Date:
DOI: https://doi.org/10.1007/BF01022593