Biotechnology Letters

, Volume 12, Issue 12, pp 899–904 | Cite as

Free trypsin-catalyzed peptide synthesis in acetonitrile with low water content

  • Václav Čeřovský


The reactions of Bz-Arg-OEt, Boc-Lys-Ome and Boc-Tyr-Lys-OMe with various amino acid amides and peptides, catalyzed by free trypsin in acetonitrile in the presence of triethylamine and 5 vol. % of water, were studied. The synthesis of Bz-Arg-Leu-NH2 was strongly dependent on the water content (from 2 to 10%) in the reaction medium. Excess of base (triethylamine) had no negative effect on the reaction. The reaction proceeded analogously also in some aliphatic alcohols but not in dimethylformamide.


Peptide Alcohol Organic Chemistry Acetonitrile Amide 
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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Václav Čeřovský
    • 1
  1. 1.Institute of Organic Chemistry and biochemistryCzechoslovak Academy of SciencesPrague 6Czechoslovakia

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